FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH610.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH610.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7670
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT376349.0612777053455No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT93412.177314211212515No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTTGATTAAAACTAAAACTTT80210.45632333767927No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT5477.1316818774445885No Hit
AGCCTAGACATCCACTGCTGACGACGACGTGGTAATGATCCTTCCGCTGT891.1603650586701435No Hit
AGCCTAGACATCCACTGCTGGTGGTAATGATCCTTCCGCTGTCTCTTATA490.6388526727509778No Hit
AGCCTAGACATCCACTGCTGCCGACGAGTGGTAATGATCCTTCCGCTGTC450.5867014341590613No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTC270.3520208604954368No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT260.3389830508474576No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC230.29986962190352023No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAGGCAAGGACTT220.28683181225554105No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGTAAAAGCAAGGACTT210.2737940026075619No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACCT190.24771838331160367No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATAAAAGCAAGGACTT190.24771838331160367No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTAAAAGCAAGGACTT190.24771838331160367No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAAAGCAAGGACTT170.2216427640156454No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT170.2216427640156454No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTAAAAGCAAGGACTT150.1955671447196871No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTAAAAGCAAGGACTT150.1955671447196871No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTAAAAGCAAGGACTT150.1955671447196871No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGGCTT140.18252933507170796No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAAAGCAAGGACTT140.18252933507170796No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGTAAAAGCAAGGACTT120.15645371577574968No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAAAGCAAGGACTT120.15645371577574968No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCAAAAGCAAGGACTT110.14341590612777053No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAGAAGCAAGGACTT110.14341590612777053No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCGAGGACTT110.14341590612777053No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAGATAAAAGCAAGGACTTT100.1303780964797914No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACTAAAAGCAAGGACTT100.1303780964797914No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT100.1303780964797914No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT100.1303780964797914No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTGAAAGCAAGGACTT100.1303780964797914No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT90.11734028683181225No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACCTT90.11734028683181225No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTAAAAGCAAGGACTT90.11734028683181225No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAGGGACTTT80.10430247718383312No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTTGAGTAAAACTAAAACTTT80.10430247718383312No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCATAAACTTT80.10430247718383312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)