FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH609.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH609.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3927
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC288573.46574993633817No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT1533.896103896103896No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTCGGAGATGTGGTAATGATCC872.2154316271963332No Hit
AGCCTAGACATCCACTGCTGCTCGGAGATGTGGTAATGATCCTTCCGCTG862.1899668958492486No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT461.1713776419658772No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTCAAAGCAAGAACT421.06951871657754No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGGCTCGGAGATGTGGTAATGAT240.6111535523300229No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC220.5602240896358543No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGCAGAGAC170.4329004329004329No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC140.35650623885918004No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC130.3310415075120957No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC120.30557677616501144No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGAACTT110.2801120448179272No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC100.2546473134708429No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC80.2037178507766743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC80.2037178507766743No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAAGCAGAGAC70.17825311942959002No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGCAGAGAC70.17825311942959002No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC70.17825311942959002No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATCTAGAATAAAAGCAGAGAC60.15278838808250572No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAGAAGCAGAGAC60.15278838808250572No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAGTAAAAGCAGAGAC60.15278838808250572No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC50.12732365673542145No Hit
AGCCTAGACATCTACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC50.12732365673542145No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT50.12732365673542145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC50.12732365673542145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTAGAATAAAAGCAGAGAC50.12732365673542145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTTAGGATTAAAACCAAAGACTT50.12732365673542145No Hit
CCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACTT50.12732365673542145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTCAAAGCAAGAACT50.12732365673542145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGAATAAAAGCAGAGAC50.12732365673542145No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAT40.10185892538833714No Hit
AGCCTAGACATCCACTGCTGCAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGACTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGACATCCATTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGACATCCACTGCTGTCGTCGGCAGCGTCAGATGTGGTAATGATC40.10185892538833714No Hit
AGCCTAGACATCCACCGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGTCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC40.10185892538833714No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC40.10185892538833714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)