FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11389
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC995987.44402493634207No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATAATAAAAGCAGAGAC900.790236192817631No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGAATAAAAGCAGAGAC450.3951180964088155No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAGGCAGAGAC380.33365528141188866No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAAAATAAAAGCAGAGAC330.28975327069979806No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGGGAC280.2458512599877074No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC230.20194924927561683No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACAATAAAAGCAGAGAC230.20194924927561683No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGGC220.1931688471331987No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGAAGAGAC210.18438844499078058No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGTAGAGAC190.16682764070594433No Hit
AGCCTAGATATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC180.1580472385635262No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCGGAGAC160.14048643427868995No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGAATAAAAGCAGAGAC160.14048643427868995No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAAAGAC140.1229256299938537No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGACT130.11414522785143559No Hit
AGCCTAGACATCCATTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.11414522785143559No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAAAC130.11414522785143559No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGAATAAAAGCAGAGAC130.11414522785143559No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGAATAAAAGCAGAGAC130.11414522785143559No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTAAACGCAAAAACTTT130.11414522785143559No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCCAGAATAAAATTAGAGAC130.11414522785143559No Hit
AGCCTAGACATCCAATGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC130.11414522785143559No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGGATAAAAGCAGAGAC120.10536482570901748No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCGAGAATAAAAGCAGAGAC120.10536482570901748No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC120.10536482570901748No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAACAGCAGAGAC120.10536482570901748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)