FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11965
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT346228.934391976598413No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTT234119.5653990806519No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTT197216.48140409527789No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT11669.745089845382365No Hit
AGCCTAGACATCCACTGCTGCCGACGATGTGGTAATGATCCTTCCGCTGT1701.420810697868784No Hit
AGCCTAGACATCCACTGCTGTCTCGTGGTAATGATCCTTCCGCTGTCTCT1561.303802758044296No Hit
AGCCTAGACATCCACTGCTGCTCGGAGATGTGGTAATGATCCTTCCGCTG1391.1617216882574175No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC1201.0029251984956122No Hit
AGCCTAGACATCCACTGCTGAGGTGGTAATGATCCTTCCGCTGTCTCTTA850.710405348934392No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT800.6686167989970748No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAAGGACT310.2590890096113665No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACCTAGTTAAAAGCAAGGACT310.2590890096113665No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT300.25073129962390306No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAAGGACT280.2340158796489762No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT260.21730045967404932No Hit
AGCCTAGACATCCACTGCTGAGAGTTGTTATCTAGTTAAAAGCAAGGACT260.21730045967404932No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAGTAAAAGCAGGGACTT240.20058503969912245No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACC210.17551190973673214No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTAAAAGCAGGGACTT210.17551190973673214No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAAGGACT200.1671541997492687No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGGCT190.15879648976180527No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGCTAAAAGCAAGGACT170.1420810697868784No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTGAAAGCAAGGACT160.13372335979941496No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTAAAAGCAGGGACTT160.13372335979941496No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAAGGACT150.12536564981195153No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAGGGACTT150.12536564981195153No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACTC150.12536564981195153No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAAGGACTT150.12536564981195153No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAAGGACT140.1170079398244881No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTGGTTAAAAGCAAGGACT140.1170079398244881No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT140.1170079398244881No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAAAGCAGGGACTT130.10865022983702466No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTAAAAGCAGGGACTC130.10865022983702466No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGAGGACT130.10865022983702466No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTACCTAGTAAAAGCAGGGACTT120.10029251984956122No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATCTAGTTAAAAGCAAGGACT120.10029251984956122No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAGGGACCT120.10029251984956122No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATCTAGTTAAAAGCAAGGACT120.10029251984956122No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTCTAGTAAAAGCAGGGACTT120.10029251984956122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)