FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7984
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT364145.60370741482966No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT140017.535070140280563No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT7279.105711422845692No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAAAGCAAGGACTT3854.822144288577154No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAATT3794.7469939879759515No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAAACTT420.5260521042084169No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTTAATTAAAAGCAAGAATT410.5135270541082164No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGAGTAAAACCAAAGACTT330.4133266533066132No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGAATAAAACCAAAGACTT290.3632264529058116No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT280.35070140280561124No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTGTAAATAAAAGCAAGGACTTT250.31312625250501No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGAACT250.31312625250501No Hit
AGCCTAGACATCCACTGCTGGTCTCGTGGTAATGATCCTTCCGCTGTCTC200.250501002004008No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGACTAAAACCAAAGACTT200.250501002004008No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTAATTAAAACCAAAGACTT190.23797595190380763No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACCT180.22545090180360722No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATTGATTAAAACCAAAGACTT160.2004008016032064No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAGCCAAAGACTT140.17535070140280562No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT130.1628256513026052No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTC130.1628256513026052No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAGGACTT120.15030060120240482No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGGTTAAAACCAAAGACTT120.15030060120240482No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATTGATTAAAACCAAAGACTT120.15030060120240482No Hit
AGCCTAGACATCCACTGCTGGAAGTTGTTATTGATTAAAACCAAAGACTT110.1377755511022044No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGGCTT110.1377755511022044No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC110.1377755511022044No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGGGCAAGGACTT110.1377755511022044No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTC100.125250501002004No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATTGATTAAAACCAAAGACTT100.125250501002004No Hit
AGCCTAGACATCCACTGCTGAAAGTCGTTATTGATTAAAACCAAAGACTT100.125250501002004No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGCCTT100.125250501002004No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTACTGATTAAAACCAAAGACTT90.11272545090180361No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT90.11272545090180361No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAGAACCAAAGACTT90.11272545090180361No Hit
AGCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTTT90.11272545090180361No Hit
AGCCTAGACATTCACTGCTGAAAGTTGTTATTGATTAAAACCAAAGACTT90.11272545090180361No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCGAAGACTT90.11272545090180361No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTAAGAGCAAGGACTT80.1002004008016032No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTTATTAAAACCAAAGACTT80.1002004008016032No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTACAACCAAAGACTT80.1002004008016032No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGCTAAAAGCAGAGACT80.1002004008016032No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATTGATTAAAACCAGAGACTT80.1002004008016032No Hit
AGCCTAGACATCCACTGCTGAAAGCTGTTATTGATTAAAACCAAAGACTT80.1002004008016032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)