FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTTCCGCTGTCTCTT12972.47191011235955No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATACTTCCGCTGTCTCTT31.6853932584269662No Hit
AGCCTAGAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACT21.1235955056179776No Hit
CGGCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGT21.1235955056179776No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATTCTTCCGCTGTCTCTT21.1235955056179776No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAAGGACTT21.1235955056179776No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT21.1235955056179776No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGAATAAAAGCAGAGAC10.5617977528089888No Hit
AGACTCGATCTCCCATCTTGACGAGTGGTGGCAGTGAACGAAAAAACATT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCCAGAGCCACGAGATCCATTGTTGAAAGT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTTCCGCTGTCTCTC10.5617977528089888No Hit
CGCCTAGACATCCCCTGCTGACGGTTGTAATGATCCTTCCTCTTTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGGAAGTTGCTATCTAGTTAAAAGCAGAGACT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACTGTGATAATGATCCTACCGCTGTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATGCTTCCGCTGTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCATTCCGCTGTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTAAGAGCAGGGACTT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCGAGAGCCAACAGATCCGTTGTTGAAACT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTAGTAATGATCCTTCCGCTGTCTCTT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAACT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTTCCACTGTCTCTT10.5617977528089888No Hit
ATACTAGAAAGCAACTAATTAAAGGATGTTGAAGTGAACGCAAAAAATGT10.5617977528089888No Hit
AGCCTAGACATACACTGCTGAAAGTTTGTGTAAGTGAAAGCAACAACTTT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCGCAAGCCAAGAGATCCGTTGTTGAAAGT10.5617977528089888No Hit
CTAATAGAAATTCACTGCTGAAAGTTTGTGGAAGTGACCGCAAAAACTTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTTTCGCTGTCTCTT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCGGGAGCCAAGAGATCCGTTGCCGAAAGT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCGAGAGCCAAGAAATCCGTTGCTAAAAGT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTTCCGTTGTCTCTT10.5617977528089888No Hit
CGGCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGCTGTTAAAAGT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGGTCCTTCCGCTGTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATTATCCTTCCGCTGTCTCCT10.5617977528089888No Hit
AGCCTAGACATGCACTGCTGACGGTGGTAATGATCCTTCCGCTGTCTCTT10.5617977528089888No Hit
ACCCCAAAACCACAACGGCGAGGGGGGGAAATATACCTTCGCTCTCTCAT10.5617977528089888No Hit
AGCCTAGACACCACTGCTGACGGTGGTAATGATCCTTCCGCTGTCTCTTA10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGAAAGTTGGTGTCAGTGATCACAAAAAAGTT10.5617977528089888No Hit
CGGAAGGATCATTACCACCGTCAGCAGTGGATGTCTAGGCTCTGTCTCTT10.5617977528089888No Hit
AGCCCAGACATCCACTGCTGACGGTGGTAATGATCCTCCCGCTGTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCCCTGCTTAACGTTTCTGGAAGTTAACTAAAAAACTTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGACGGTGGTAATGATCCTCCCGCTGTCTCTT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAGGACT10.5617977528089888No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGAAGAGACT10.5617977528089888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)