FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n02_repeat-ITS-OOATH575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n02_repeat-ITS-OOATH575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT1158568.00305235970885No Hit
AGCCTAGACATCCACTGCTGAAAGTTGCTATCTAGTTAAAAGCAGAGACT329119.317915003521954No Hit
AGCCTAGACATCCACTGCTGAAAGTTTGTGGAAGTGAACGCAAAAACTTT670.39328480864052595No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTATTTAAAAGCAGAGACT610.3580652735383893No Hit
AGCCTAGACATCCACTGCTAAAAGTTGTTATCTAGTTAAAAGCAGAGACT590.3463254285043437No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAATTAAAAGCAGAGACT390.22892697816388827No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACC350.20544728809579715No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAGGCAGAGACT350.20544728809579715No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAAACT320.18783752054472883No Hit
CGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT310.181967598027706No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGGGACT300.17609767551068325No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGGCT260.15261798544259217No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCGGAGACT260.15261798544259217No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAAAGACT220.12913829537450106No Hit
AGCCTAGACATCCACTGCTAAAAGTTGCTATCTAGTTAAAAGCAGAGACT220.12913829537450106No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGAAT220.12913829537450106No Hit
AGCCTATACATCCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT220.12913829537450106No Hit
AGCCTAGACATCCACTGCTGAAGGTTGCTATCTAGTTAAAAGCAGAGACT200.11739845034045551No Hit
AGCCTAGACATCCACTGCTGAAGGTTGTTATCTAGTTAAAAGCAGAGACT200.11739845034045551No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCTACTTAAAAGCAGAGACT190.11152852782343273No Hit
AGCCTAGACATCCACTGCTGACAGTTGTTATCTAGTTAAAAGCAGAGACT180.10565860530640996No Hit
AGCCTAGACATCCCCTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACT180.10565860530640996No Hit
AGCCTAGACATCACTGCTGAAAGTTGTTATCTAGTTAAAAGCAGAGACTT180.10565860530640996No Hit
AGCCTAGACATCCACTGCTGAAAGTTGTTATCGAGTTAAAAGCAGAGACT180.10565860530640996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)