FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOChi749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOChi749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA31414.055505819158459No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGAATCTGTACTT30913.831692032229185No Hit
CGGAAGGATCATTACCACAGCAGTGGATGTCTAGGCTCTGTCTCTTATAC22910.250671441360788No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCAGTGGATGTCTAGGCTCTGTC1466.535362578334826No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT893.9838854073410923No Hit
CGGAAGGATCATTACCACATCTCCGCAGCAGTGGATGTCTAGGCTCTGTC883.9391226499552374No Hit
CGGAAGGATCATTACCACGAGACCCAGCAGTGGATGTCTAGGCTCTGTCT843.7600716204118174No Hit
CGGAAGGATCATTACCACATCCAGCAGTGGATGTCTAGGCTCTGTCTCTT823.670546105640107No Hit
CGGAAGGATCATTACCACATCTCCCAGCAGTGGATGTCTAGGCTCTGTCT813.6257833482542523No Hit
CGGAAGGATCATTACCACATCTCAGCAGTGGATGTCTAGGCTCTGTCTCT803.5810205908683974No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCGTATCAAC783.491495076096687No Hit
CGGAAGGATCATTACCACACCAAAAAACTTACCACGTGAATCTGTACTTT562.5067144136078783No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCGTTAT411.8352730528200536No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC301.342882721575649No Hit
CGGAAGGATCATTACCACTGTCTCTTATACACACAGCAGTGGATGTCTAG231.0295434198746642No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCTGGTA120.5371530886302597No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG110.4923903312444047No Hit
CGGAAGGATCATTACCACAGCAGTGGATGTCTAGGCTCTGTCCCTTATAC100.4476275738585497No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT90.40286481647269473No Hit
CGGAAGGATCATTACCACCGTCGGCAGCAGTGGATGTCTAGGCTCTGTCT80.35810205908683973No Hit
CGGAAGGATCATTACCACACCAAAAAACCTTACCACGTGAATCTGTACTT70.3133393017009848No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCTCTGTCTC70.3133393017009848No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATTCA60.26857654431512984No Hit
CGGAAGGATCATTACCACACCCAGCAGTGGATGTCTAGGCTCTGTCTCTT50.22381378692927484No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCGTATCAAA50.22381378692927484No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGAACCGTATCAA50.22381378692927484No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGGATCTGTACTT50.22381378692927484No Hit
CGGAAGGATCATTACCACACCTCAGCAGTGGATGTCTAGGCTCTGTCTCT40.17905102954341987No Hit
CGGAAGGATCATTACCACACCTAAAAGCTTTCCACGTGAACCGTATCAAA40.17905102954341987No Hit
CGGAAGGATTATTACCACACCAAAAAAACTTACCACGTGAATCTGTACTT30.13428827215756492No Hit
CGGAAGGATCATTACCACAGCAGTGGATGTCTAGGCTCTGTCTCTTAACA30.13428827215756492No Hit
CGGAAGGATCATTACCACACCTCCCAGCAGTGGATGTCTAGGCTCTGTCT30.13428827215756492No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGCGAATCTGTACTT30.13428827215756492No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTACCACGTGAATCTGTACTT30.13428827215756492No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAA30.13428827215756492No Hit
CGGAAGGATCATTACCCCACCAAAAAAACTTACCACGTGAATCTGTACTT30.13428827215756492No Hit
CGGAAGGATCATTACCACATCTCCCAGCAGTGAATGTCTAGGCTCTGTCT30.13428827215756492No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCAGTAGATGTCTAGGCTCTGTC30.13428827215756492No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGACTCTGTACTT30.13428827215756492No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAA30.13428827215756492No Hit
CGGAAGGATCATTACCACACCTAAAAACTTACCACGTGAATCTGTACTTT30.13428827215756492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)