FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOChi727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOChi727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12260
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT437035.64437194127243No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC234519.127243066884176No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGTCAGAAAT9357.626427406199021No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTTCCACGTGAACCGTTGTAA7656.239804241435563No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA2351.9168026101141926No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT1771.4437194127243067No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT820.66884176182708No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTTCCACGTGAACCGTTGAAA590.4812398042414356No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCTACGTGAACCGTATCAAC560.4567699836867863No Hit
CGGAAGGATCATTACCACCAAAAACTATCCACGTGAACCACTTTGTATTG500.40783034257748774No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTTCCACGTGAACCGTTGCAA470.3833605220228385No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGAATCTGTACTG450.367047308319739No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATGAAC420.3425774877650897No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTCTGTAT390.3181076672104404No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC340.27732463295269166No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT330.26916802610114193No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAC310.2528548123980424No Hit
CGGAAGGATCATTACCACACCAAAAACCATCCACGTGAACCGTCAGAAAT300.24469820554649263No Hit
CGGAAGGATCATTACCACACCAAAGACTATCCACGTGAACCACTTTGTAT290.2365415986949429No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT280.22838499184339314No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT270.2202283849918434No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT260.21207177814029365No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGTCAGAAGT260.21207177814029365No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCACTTTGTAT260.21207177814029365No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT260.21207177814029365No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT250.20391517128874387No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT230.18760195758564438No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT230.18760195758564438No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT220.17944535073409462No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT220.17944535073409462No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTCGTAT220.17944535073409462No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT220.17944535073409462No Hit
CGGAAGGATCATTACCACCTAAAAACTTTCCACGTGAACCGTATCAACAA200.1631321370309951No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTACCAAC200.1631321370309951No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA190.15497553017944535No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAC190.15497553017944535No Hit
CGGAAGGATCATTACCACCAAAAACAATCCACGTGAACCGTCAGAAATAG180.1468189233278956No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT170.13866231647634583No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATCAAC170.13866231647634583No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTACACGTGAACCGTATCAAC170.13866231647634583No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC170.13866231647634583No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT160.13050570962479607No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAA160.13050570962479607No Hit
CAGTGGATGTCTAGGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.13050570962479607No Hit
CGGAAGGATCATTACCACACCTAAAAACTTGCCACGTGAACCGTATCAAC160.13050570962479607No Hit
CGGAAGGATCATTACCACACCGAAAACTATCCACGTGAACCACTTTGTAT150.12234910277324632No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA140.11419249592169657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT140.11419249592169657No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCGTCAGATGTGTATAAGAGACA140.11419249592169657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTCAGAAAT140.11419249592169657No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAC140.11419249592169657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCAATTTGTAT140.11419249592169657No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGCCAGAAAT140.11419249592169657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCAAGTGAACCACTTTGTAT130.10603588907014683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)