FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOChi717.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOChi717.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGAATCTGTACTT46415.394824153948242No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC45515.096217650962176No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCGTTAT34311.380225613802256No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCTCTGTCTC30910.252156602521566No Hit
CGGAAGGATCATTACCACATCTCCGAGCCACGAGACCAGCAGTGGATGTC2377.863304578633046No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTACATA1836.071665560716656No Hit
CGGAAGGATCATTACCACATCGTCGGCAGCAGTGGATGTCTAGGCTCTGT1454.810882548108825No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCAGTGGATGTCTAGGCTCTGTC1304.313205043132051No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT752.488387524883875No Hit
CGGAAGGATCATTACCACACCAAAAAACTTACCACGTGAATCTGTACTTT160.53085600530856No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG130.43132050431320507No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCCCTGTCTC120.3981420039814201No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGAATCTGTACTC110.36496350364963503No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCTGGTA90.29860650298606506No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACTGTCGTTAT80.26542800265428No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTAAACA80.26542800265428No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTATATA70.232249502322495No Hit
CGGAAGGATCATTACCACGAGACCAGCCGTGGATGTCTAGGCTCTGTCTC70.232249502322495No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC70.232249502322495No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA70.232249502322495No Hit
CGGAAGGATCATTACCACATCGTCGGCAGCAGTGGATGTCTCGGCTCTGT60.19907100199071004No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTAACACGTGAATCTGTACTT60.19907100199071004No Hit
CGGAAGGATCATTACCACACCGTCGGCAGCAGTGGATGTCTAGGCTCTGT60.19907100199071004No Hit
CGGAAGGATCATTACCACACCTAAAAAATTTCCACGTGAACCGTATCAAC50.16589250165892502No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGAACCTGTACTT50.16589250165892502No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATTCA50.16589250165892502No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGCGAATCTGTACTT40.13271400132714No Hit
CGGAAGGATCATTACCACGAGACCAACAGTGGATGTCTAGGCTCTGTCTC40.13271400132714No Hit
CGGAAGGATCATTACCACGGGACCAGCAGTGGATGTCTAGGCTCTGTCTC40.13271400132714No Hit
CGGAAGGATCATTACCACCAAAAAAACTTACCACGTGAATCTGTACTTTC40.13271400132714No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACTGTCGTTAT40.13271400132714No Hit
CGGAAGGATCATTACCACGAAACCAGCAGTGGATGTCTAGGCTCTGTCTC40.13271400132714No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACTGTCGTTAT40.13271400132714No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTCGTTAT40.13271400132714No Hit
CGAAGGATCATTATCACACCAAAAAACTATCCACGTGAACCGTTACATAC40.13271400132714No Hit
CGGAAGGATCATTACCACACCAAAAAAACTTACCACGTGACTCTGTACTT40.13271400132714No Hit
CGGAAGGATCATTACCACACCAAAAAACCTTACCACGTGAATCTGTACTT40.13271400132714No Hit
CGGAAGGATCATTACCACCTAAAAACTTTCCACGTGAACTGTCGTTATTT40.13271400132714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)