FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOChi691.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOChi691.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5346
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTATCAAA196836.81257014590348No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC67912.701084923307146No Hit
CGGAAGGATCATTACCACACCAAAAACAACCCACGTGAAAATGTACTGTC3746.995884773662551No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCTCTGTCTC2685.013093901982791No Hit
CGGAAGGATCATTACCACATCTCCAGCAGTGGATGTCTAGGCTCTGTCTC2003.7411148522259636No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCAGTGGATGTCTAGGCTCTGTC1793.3482977927422373No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATTCA1522.843247287691732No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT1192.225963337074448No Hit
CGGAAGGATCATTACCACATCTCCGAGCCCACGAGACTCTCTATGATCTC961.7957351290684627No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCCCGTGAACCGTATCAAC661.2345679012345678No Hit
CGGAAGGATCATTACCACTCAGCAGTGGATGTCTAGGCTCTGTCTCTTAT601.122334455667789No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACTGTATCAAA460.8604564160119716No Hit
CGGAAGGATCATTACCACTGTCTCTTATACACAGCAGTGGATGTCTAGGC460.8604564160119716No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCGTCAGATGTGTATAAGAGACA310.5798728020950242No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTAAACA310.5798728020950242No Hit
CGGAAGGATCATTACCACATCTCCGAGCCCACGAGACCCAGCAGTGGATG240.44893378226711567No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCAACGTGAACTGTATCAAA210.3928170594837262No Hit
CAGTGGATGTCTAGGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA200.3741114852225963No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACCGTATCAAA170.3179947624392069No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTGTCAAC160.29928918817807704No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCACGTGAACTGTATCAAAA160.29928918817807704No Hit
CGGAAGGATCATTACCACACCTAAAAAACCTCCACGTGAACTGTATCAAA140.2618780396558174No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAGCTGTATCAAA140.2618780396558174No Hit
CGGAAGGATCATTACCACCTAAAAAACTTCCACGTGAACTGTATCAAAAC140.2618780396558174No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTACCAAA120.22446689113355783No Hit
CGGAAGGATCATTACCACACCAAAAACCACCCACGTGAAAATGTACTGTC110.205761316872428No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGCGAACTGTATCAAA90.16835016835016833No Hit
CGGAAGGATCATTACCCCACCTAAAAAACTTCCACGTGAACTGTATCAAA80.14964459408903852No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTATCAAC80.14964459408903852No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTATCAAG80.14964459408903852No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTATTAAA70.1309390198279087No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGGACTGTATCAAA70.1309390198279087No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC70.1309390198279087No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC60.11223344556677892No Hit
CGGAAGGATCATTACCACGCCTAAAAAACTTCCACGTGAACTGTATCAAA60.11223344556677892No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCAGTAGATGTCTAGGCTCTGTC60.11223344556677892No Hit
CGGAAGGATTATTACCACACCTAAAAAACTTCCACGTGAACTGTATCAAA60.11223344556677892No Hit
CGGAAGGATCATTACCACACCTAAAGAACTTCCACGTGAACTGTATCAAA60.11223344556677892No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC60.11223344556677892No Hit
CGGAAGGATCATTACCACACCTAAGAAACTTCCACGTGAACTGTATCAAA60.11223344556677892No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTCCACGTGAACTGTATCGAA60.11223344556677892No Hit
CGGAAGGATCATTACCACAGCAGTGGATGTCTAGGCTCTGTCTCTTATAC60.11223344556677892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)