FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13847
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT1249390.22170867335885No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT1641.184372066151513No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT420.303314797429046No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT400.28887123564671047No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT360.2599841120820394No Hit
CGGAAGGATCATTACCACATCTCCGAGCAGCAGTGGATGTCTAGGCTCTG340.2455405502997039No Hit
CGGAAGGATCATTACCACGACAGCAGTGGATGTCTAGGCTCTGTCTCTTA290.2094316458438651No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT220.15887917960569076No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT210.151657398714523No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT190.13721383693218747No Hit
TGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT180.1299920560410197No Hit
CGGAAGGATCATACCACACCAAAAACTATCCACGTGAACCACTTTGTATT170.12277027514985195No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT170.12277027514985195No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCCCGTGAACCACTTTGTAT160.11554849425868419No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT150.10832671336751643No Hit
CGGAAGGATCATTATCACACCAAAAACTATCCACGTGAACCACTTTGTAT140.10110493247634866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)