FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT78351.68316831683168No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC40626.798679867986795No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA281.8481848184818481No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA221.4521452145214522No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT161.056105610561056No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT100.6600660066006601No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCACTTTGTA70.46204620462046203No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT60.39603960396039606No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT60.39603960396039606No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTACACGTGAACCGTATCAAC40.264026402640264No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC40.264026402640264No Hit
CGGAAGGATCATTACCACACCAAAAAATATCCACGTGAACCACTTTGTAT40.264026402640264No Hit
CGGAAGGATCATTACCACACCAAAAACTTTCCACGTGAACCGTATCAACA40.264026402640264No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATAAAC40.264026402640264No Hit
CGGAAGGATCATTACCACACCAAAAACTTTCCACGTGAACCACTTTGTAT40.264026402640264No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACGTTGTAT30.19801980198019803No Hit
TGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC30.19801980198019803No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTCTGTAT30.19801980198019803No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT30.19801980198019803No Hit
CGGAAGGATCATTACCACACCAAAAGCTATCCACGTGAACCACTTTGTAT30.19801980198019803No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCAAGTGAACCACTTTGTAT30.19801980198019803No Hit
CGGAAGGATCATTACCACACCAAAAACTATTCACGTGAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACGGTATCAAC20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAACCTTTCCACGTGAACCGTATCAAC20.132013201320132No Hit
CGGAAGGATCATACCACACCAAAAACTATCCACGTGAACCACTTTGTATT20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT20.132013201320132No Hit
TGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACAACACCTAAAAACTTTCCACGTGAACCGTATCAAC20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACAACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAC20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA20.132013201320132No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAAACACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCTGGTA20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACATGAACCGTATCAAC20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAAACTTTCCACGTGAACCGTATCAAC20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTTAACCACTTTGTAT20.132013201320132No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCGAC20.132013201320132No Hit
CGGAAGGATCATTACCACACCAAAAACTGTCCACGTGAACCACTTTGTAT20.132013201320132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)