FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT329164.98815165876776No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC94818.72037914691943No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA561.1058451816745656No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA430.84913112164297No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT420.8293838862559242No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT220.4344391785150079No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCACTTTGTA210.4146919431279621No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT170.33570300157977884No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT140.2764612954186414No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT130.2567140600315956No Hit
CGGAAGGATCATTACCACACCAAAAACTTTCCACGTGAACCGTATCAACA120.23696682464454977No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT120.23696682464454977No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT110.21721958925750395No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT110.21721958925750395No Hit
CGGAAGGATCATTACCACACCAAAGACTATCCACGTGAACCACTTTGTAT110.21721958925750395No Hit
CGGAAGGATCATTACCACACCAAAAGCTATCCACGTGAACCACTTTGTAT100.1974723538704581No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGC90.17772511848341233No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT90.17772511848341233No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT90.17772511848341233No Hit
CGGAAGGATCATTACCACCAAAAACTATCCACGTGAACCACTTTGTATTG90.17772511848341233No Hit
CGGAAGGATCATTACCACACCAAAAACTGTCCACGTGAACCACTTTGTAT90.17772511848341233No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT80.1579778830963665No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT70.1382306477093207No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT70.1382306477093207No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC70.1382306477093207No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT70.1382306477093207No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTCGTAT70.1382306477093207No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCCCGTGAACCACTTTGTAT70.1382306477093207No Hit
CAGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT70.1382306477093207No Hit
CGGAAGGATCATTACCACACCAAAACTATCCACGTGAACCACTTTGTATT60.11848341232227488No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT60.11848341232227488No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCAATTTGTAT60.11848341232227488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)