FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13358
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT972772.81778709387633No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA6274.693816439586764No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT1841.3774517143284921No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC1010.7561012127564006No Hit
CGGAAGGATCATTACCACCAAAAACTATCCACGTGAACCACTTTGTATTG730.5464889953585866No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT640.4791136397664321No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT560.41922443479562804No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT540.40425213355292705No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT460.34436292858212303No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT460.34436292858212303No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT400.29944602485402005No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT400.29944602485402005No Hit
CGGAAGGATCATTACCACACCGGCAGCAGTGGATGTCTAGGCTCTGTCTC390.29195987423266956No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT380.28447372361131906No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTCGTAT340.2545291211259171No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAAAC330.24704297050456656No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAAACACTTTGTAT300.22458451864051507No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT300.22458451864051507No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCCCGTGAACCACTTTGTAT290.21709836801916457No Hit
CGGAAGGATCATTACCACACCAAAAGCTATCCACGTGAACCACTTTGTAT290.21709836801916457No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT290.21709836801916457No Hit
CGGAAGGATCATTACCACACCAAAAACTATTCACGTGAACCACTTTGTAT280.20961221739781402No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC280.20961221739781402No Hit
CGGAAGGATCATTACCACACCGAAAACTATCCACGTGAACCACTTTGTAT280.20961221739781402No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT270.20212606677646353No Hit
CGGAAGGATCATTACAACACCAAAAACTATCCACGTGAACCACTTTGTAT260.19463991615511306No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTCTGTAT250.18715376553376253No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCAATTTGTAT250.18715376553376253No Hit
CGGAAGGATCATTACCACACCAAAGACTATCCACGTGAACCACTTTGTAT250.18715376553376253No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT230.17218146429106151No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGCAT230.17218146429106151No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACTACTTTGTAT230.17218146429106151No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT220.16469531366971102No Hit
CGGAAGGATCATTACCACACCAAAACTATCCACGTGAACCACTTTGTATT220.16469531366971102No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCAAGTGAACCACTTTGTAT220.16469531366971102No Hit
CGGAAGGATCATTACCACACCAAAAACCATCCACGTGAACCACTTTGTAT210.15720916304836052No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTGT210.15720916304836052No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT210.15720916304836052No Hit
CGGAAGGATCATTACCAAACCAAAAACTATCCACGTGAACCACTTTGTAT200.14972301242701003No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAC190.14223686180565953No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT180.13475071118430904No Hit
CGGAAGGATCATTACCACACCAAAAACTATCTACGTGAACCACTTTGTAT180.13475071118430904No Hit
CGGAAGGATCATTACCACACCAAAAACTGTCCACGTGAACCACTTTGTAT180.13475071118430904No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT170.12726456056295854No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACAACTTTGTAT150.11229225932025753No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACGTTGTAT150.11229225932025753No Hit
CGGAAGGATCATTATCACACCAAAAACTATCCACGTGAACCACTTTGTAT150.11229225932025753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)