FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7919
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC496162.64679883823715No Hit
CGGAAGGATCATTACCACACCAAACAACATCCACGTGAACCGATTTATGG5016.32655638338174No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATTCA2813.5484278317969444No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC1581.99520141431999No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTAAACA821.0354842783179694No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC670.846066422528097No Hit
CGGAAGGATCATTACCACATCTCCGAGCCCACGAGACGTCTGCTAATCTC520.6566485667382246No Hit
CGGAAGGATCATTACCACTCGTCGGCAGCGTCAGCAGTGGATGTCTAGGC440.5556257103169592No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT420.5303699962116428No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATTCAA370.4672307109483521No Hit
CGGAAGGATCATTACCACACCTAAAAAACTTTCCACGTGAACCGTATCAA320.40409142568506123No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT310.3914635686324031No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCTCTGTCTC300.37883571157974494No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC270.34095214042177047No Hit
CGGAAGGATCATTACCACACCGACCACAGCAGTGGATGTCTAGGCTCTGT260.3283242833691123No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGGACCGTATCAAC260.3283242833691123No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAC250.3156964263164541No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTCGTTAT250.3156964263164541No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAC240.30306856926379594No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATCAAC230.29044071221113776No Hit
CGGAAGGATCATTACCACCTAAAAACTTTCCACGTGAACCGTATCAACAA230.29044071221113776No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC210.2651849981058214No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC210.2651849981058214No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTTGTAAT200.2525571410531633No Hit
CGGAAGGATCATTACCACACCTAAAAGCTTTCCACGTGAACCGTATCAAC200.2525571410531633No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC200.2525571410531633No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCGAC190.23992928400050512No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAC180.22730142694784694No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATGAAC170.2146735698951888No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACAGTATCAAC170.2146735698951888No Hit
CGGAAGGATCATTACCACATCTCCGAGCCCACGAGACAGCAGTGGATGTC160.20204571284253062No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATAAAC160.20204571284253062No Hit
CGGAAGGATCATTACCACACCTAAAGACTTTCCACGTGAACCGTATCAAC150.18941785578987247No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGC150.18941785578987247No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTATCAAC150.18941785578987247No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGCGAACCGTATCAAC140.1767899987372143No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGATTTATG130.16416214168455615No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTAAACCGTATCAAC120.15153428463189797No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTGTCAAC120.15153428463189797No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA120.15153428463189797No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCTACGTGAACCGTATCAAC110.1389064275792398No Hit
CGGAAGGATCATTACCACACCTAAAACTTTCCACGTGAACCGTATCAACA110.1389064275792398No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATTAAC110.1389064275792398No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTACCAAC110.1389064275792398No Hit
CGGAAGGATCATTACCACACCTAGAAACTTTCCACGTGAACCGTATCAAC100.12627857052658165No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGCATCAAC100.12627857052658165No Hit
CGGAAGGATCATTATCACACCTAAAAACTTTCCACGTGAACCGTATCAAC100.12627857052658165No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTACACGTGAACCGTATCAAC90.11365071347392347No Hit
CGGAAGGATCATTACCACACCTAAGAACTTTCCACGTGAACCGTATCAAC90.11365071347392347No Hit
CGGAAGGATCATTACCACACCTGAAAACTTTCCACGTGAACCGTATCAAC90.11365071347392347No Hit
CGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAACA90.11365071347392347No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCGACGTGAACCGTATCAAC90.11365071347392347No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCCAC90.11365071347392347No Hit
CGGAAGGATCATACCACACCTAAAAACTTTCCACGTGAACCGTATCAACA80.10102285642126531No Hit
CGGAAGGATCATTACCACATCTCCGAGCCCACGAGACCAGCAGTGGATGT80.10102285642126531No Hit
CGGAAGGATCATTACCACAACTAAAAACTTTCCACGTGAACCGTATCAAC80.10102285642126531No Hit
CGGAAGGATCATTACCAAACCTAAAAACTTTCCACGTGAACCGTATCAAC80.10102285642126531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)