FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15039
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC642342.708956712547376No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT379925.260988097612874No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA11487.633486269033845No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGTCAGAAAT9516.323558747257131No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA810.5385996409335727No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC800.5319502626504422No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT740.492053992951659No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT590.3923133187047011No Hit
CGGAAGGATCATTACCACGAGACACCAGCAGTGGATGTCTAGGCTCTGTC540.3590664272890485No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC310.20613072677704633No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACC290.1928319702107853No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT290.1928319702107853No Hit
CGGAAGGATCATTACCACACCAAAAACCATCCACGTGAACCGTCAGAAAT280.18618259192765477No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAC280.18618259192765477No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC280.18618259192765477No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTAAACA270.17953321364452424No Hit
CGGAAGGATCATTACCACACCTAAAGACTTTCCACGTGAACCGTATCAAC240.15958507879513265No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC240.15958507879513265No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGCATCAAC220.1462863222288716No Hit
CGGAAGGATCATTACCACACCAAAAAAACTATCCACGTGAACCGTCAAAA220.1462863222288716No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCGAC220.1462863222288716No Hit
CGGAAGGATCATTACCACACCTAAAAGCTTTCCACGTGAACCGTATCAAC220.1462863222288716No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCACTTTGTA210.13963694394574108No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTACCAAC210.13963694394574108No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAC200.13298756566261055No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC200.13298756566261055No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGGACCGTATCAAC200.13298756566261055No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGCGAACCGTATCAAC190.12633818737948002No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC190.12633818737948002No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTGTCAAC190.12633818737948002No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT180.11968880909634949No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAC170.11303943081321897No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT170.11303943081321897No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTCAGAAA170.11303943081321897No Hit
CGGAAGGATCATTACCACCTAAAAACTTTCCACGTGAACCGTATCAACCC160.10639005253008844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)