FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12981
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC372328.68037901548417No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTTGTAC283721.85501887373854No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTAACCAA137010.553886449426084No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTAAGCAA10878.373777058778215No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT6354.891764887142747No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTAATCAA1681.2941992142361913No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTTGTA1020.785763808643402No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTTCACGTGAACCGTTTAATA880.6779138741237193No Hit
CGGAAGGATCATTACCACACCTAAAAACCTATCCACGTGAACCGTTAAGC830.6393960403666897No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC670.5161389723441954No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATACACGTGAACCGTTAAGC590.45451043833294813No Hit
CGGAAGGATCATTACCACACCAAAAAACTATCCACGTGAACCGTTACATA570.4391033048301364No Hit
CGGAAGGATCATTACCACATCCAGCAGTGGATGTCTAGGCTCTGTCTCTT570.4391033048301364No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGGACCGTTTGTAC430.33125337031045377No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAACC430.33125337031045377No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA390.30043910330483015No Hit
CGGAAGGATCATTACCACTGTCTCTTACAGCAGTGGATGTCTAGGCTCTG390.30043910330483015No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTAAGCA380.2927355365534242No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGTTTGTACC370.28503196980201834No Hit
CGGAAGGATCATTACCACACCTGAAAACTATCCACGTGAACCGTTTGTAC320.24651413604498884No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCAACGTGAACCGTTTGTAC320.24651413604498884No Hit
CGGAAGGATCATTACCACACCAAAAACAATCCACGTGAACCGTCAGAAAT280.21569986903936522No Hit
CGGAAGGATCATTACCACGCCTAAAAAACTATCCACGTGAACCGTTAAGC270.20799630228795934No Hit
CGGAAGGATCATTACCACACCTAAAAAACTAACCACGTGAACCGTTAAGC250.19258916878514754No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCATGTGAACCGTTCTAC240.18488560203374163No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTTAAGCAA220.16947846853092982No Hit
CGGAAGGATCATTACCACACCTAAAAAACTACCCACGTGAACCGTTAAGC220.16947846853092982No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT220.16947846853092982No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAGCCGTTAAGC200.154071335028118No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTTAACCAA200.154071335028118No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCGTTAAGCA190.1463677682767121No Hit
CGGAAGGATTATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC180.13866420152530623No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGCGAACCGTTTGTAC170.13096063477390033No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAGCCGTTTGTAC170.13096063477390033No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCACTTTGT170.13096063477390033No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGACCCGTTAAGC160.12325706802249442No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTGTAAC160.12325706802249442No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCGCTAACCAA160.12325706802249442No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCGTTTGTAC160.12325706802249442No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTTAACCA150.11555350127108852No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGCGAACCGTTAAGC140.10784993451968261No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCCTTTGTAC130.1001463677682767No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT130.1001463677682767No Hit
CGGAAGGATCATTACCACACCTTAAAACTATCCACGTGAACCGTCTGTAC130.1001463677682767No Hit
CGGAAGGATCATTACCACACCTAGAAAACTATCCACGTGAACCGTTAAGC130.1001463677682767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)