FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16391
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT1363983.21029833445183No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT1951.189677261912025No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC1891.1530718076993471No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT530.3233481788786529No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT490.2989445427368678No Hit
CGGAAGGATCATTACCACCAAAAACTATCCACGTGAACCACTTTGTATTG490.2989445427368678No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT480.2928436337014215No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT480.2928436337014215No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTCTGTAT410.25013727045329753No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT410.25013727045329753No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT410.25013727045329753No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT400.24403636141785123No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT390.237935452382405No Hit
CGGAAGGATCATTACCACACCGAAAACTATCCACGTGAACCACTTTGTAT380.2318345433469587No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT360.21963272527606614No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT360.21963272527606614No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTCGTAT360.21963272527606614No Hit
CGGAAGGATCATTACCACACCAAAAGCTATCCACGTGAACCACTTTGTAT360.21963272527606614No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT350.21353181624061984No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT350.21353181624061984No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT340.20743090720517354No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCCCGTGAACCACTTTGTAT330.2013299981697273No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAC300.18302727106338845No Hit
CGGAAGGATCATTACCACACCAAAACTATCCACGTGAACCACTTTGTATT300.18302727106338845No Hit
CGGAAGGATCATTACCACACCAAAAACTATTCACGTGAACCACTTTGTAT290.17692636202794215No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT270.1647245439570496No Hit
CGGAAGGATCATTACCACACCAAAAACTGTCCACGTGAACCACTTTGTAT270.1647245439570496No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCATGTGAACCACTTTGTAT230.1403209078152645No Hit
CGGAAGGATCATTACCACACCAGAAACTATCCACGTGAACCACTTTGTAT220.1342199987798182No Hit
CGGAAGGATCATTATCACACCAAAAACTATCCACGTGAACCACTTTGTAT220.1342199987798182No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTGT210.12811908974437192No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTTT210.12811908974437192No Hit
CGGAAGGATCATTACCACACCAAAAACCATCCACGTGAACCACTTTGTAT200.12201818070892562No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAATCACTTTGTAT190.11591727167347934No Hit
CGGAAGGATCATACCACACCAAAAACTATCCACGTGAACCACTTTGTATT180.10981636263803307No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGCAT170.10371545360258677No Hit
TGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT170.10371545360258677No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAAACACTTTGTAT170.10371545360258677No Hit
CGGAAGGATCATTACCACACCAAAAACTATCTACGTGAACCACTTTGTAT170.10371545360258677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)