FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10491
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT696866.4188351920694No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC137713.125536173863312No Hit
CGGAAGGATCATTACCACGGTCGTCAGCAGTGGATGTCTAGGCTCTGTCT2051.9540558574015825No Hit
CGGAAGGATCATTACCACAGCAGTGGATGTCTAGGCTCTGTCTCTTATAC1521.4488609284148317No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT880.8388142217138499No Hit
CGGAAGGATCATTACCACCAAAAACTATCCACGTGAACCACTTTGTATTG580.5528548279477647No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT450.42893909064912783No Hit
CGGAAGGATCATTACCACACCAAAGACTATCCACGTGAACCACTTTGTAT380.36221523210370793No Hit
CGGAAGGATCATTACCACACCAAAAGCTATCCACGTGAACCACTTTGTAT380.36221523210370793No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT340.32408731293489657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT340.32408731293489657No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT320.3050233533504909No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTCTGTAT280.26689543418167955No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGCAT270.25736345438947666No Hit
CGGAAGGATCATTACCACACCAAAAACTGTCCACGTGAACCACTTTGTAT270.25736345438947666No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT260.24783147459727387No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT250.238299494805071No Hit
CGGAAGGATCATTACCACACCAAGAACTATCCACGTGAACCACTTTGTAT240.22876751501286818No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAC240.22876751501286818No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT220.20970355542846247No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT220.20970355542846247No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT190.18110761605185396No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTGT190.18110761605185396No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT170.16204365646744828No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC170.16204365646744828No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTCGTAT170.16204365646744828No Hit
CGGAAGGATCATTACCCCACCAAAAACTATCCACGTGAACCACTTTGTAT160.15251167667524546No Hit
CGGAAGGATCATTACCACACCAAAAACCATCCACGTGAACCACTTTGTAT140.13344771709083977No Hit
CGGAAGGATCATTACCACACCGAAAACTATCCACGTGAACCACTTTGTAT140.13344771709083977No Hit
CGGAAGGATCATACCACACCAAAAACTATCCACGTGAACCACTTTGTATT130.12391573729863693No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT120.11438375750643409No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT120.11438375750643409No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT120.11438375750643409No Hit
CGGAAGGATCATTACCATACCAAAAACTATCCACGTGAACCACTTTGTAT110.10485177771423124No Hit
CGGAAGGATCATTACCACACCAGAAACTATCCACGTGAACCACTTTGTAT110.10485177771423124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)