FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7401
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA583478.82718551547089No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAA751.0133765707336846No Hit
CGGAAGGATCATTACCACGTCGTCGGCAGCAGTGGATGTCTAGGCTCTGT630.8512363194162952No Hit
CGGAAGGATCATTACCACGAGACCCAGCAGTGGATGTCTAGGCTCTGTCT610.8242129441967302No Hit
CGGAAGGATCATTACCACGAGACCAGCAGTGGATGTCTAGGCTCTGTCTC440.5945142548304283No Hit
CGGAAGGATCATTACCACGAGACAGCAGTGGATGTCTAGGCTCTGTCTCT430.5810025672206459No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAA390.526955816781516No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGGACCGTATCAAA390.526955816781516No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAG380.5134441291717335No Hit
CGGAAGGATCATTACCACCTAAAAACTTTCCACGTGAACCGTATCAAACC350.47290906634238616No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAA330.4458856911228212No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCGAA310.4188623159032563No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGCGAACCGTATCAAA300.40535062829347385No Hit
CGGAAGGATCATTACCACACCTAAAAGCTTTCCACGTGAACCGTATCAAA290.39183894068369135No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATCAAA260.351303877854344No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA260.351303877854344No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAA260.351303877854344No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTACCAAA260.351303877854344No Hit
CGGAAGGATCATTACCACACCCAAAAACTTTCCACGTGAACCGTATCAAA250.33779219024456153No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGA240.3242805026347791No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAA240.3242805026347791No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAA200.2702337521956492No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC180.24321037697608433No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAA170.22969868936630186No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGCATCAAA160.2161870017565194No Hit
CGGAAGGATCATTACCCCACCTAAAAACTTTCCACGTGAACCGTATCAAA160.2161870017565194No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCATATCAAA160.2161870017565194No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTAAACCGTATCAAA150.20267531414673692No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTGTCAAA150.20267531414673692No Hit
CGGAAGGATCATTACCACACCTAAGAACTTTCCACGTGAACCGTATCAAA140.18916362653695445No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACAGTATCAAA140.18916362653695445No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACTGTATCAAA140.18916362653695445No Hit
CGGAAGGATCATTACCACACCTAAAACTTTCCACGTGAACCGTATCAAAC130.175651938927172No Hit
CGGAAGGATCATTACCACACCTAAAGACTTTCCACGTGAACCGTATCAAA120.16214025131738954No Hit
CGGAAGGATCATTACCACACCTAGAAACTTTCCACGTGAACCGTATCAAA120.16214025131738954No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCATGTGAACCGTATCAAA110.14862856370760708No Hit
TGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA100.1351168760978246No Hit
CGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAAC90.12160518848804217No Hit
CGGAAGGATCATTACCACACCTAAAAATTTTCCACGTGAACCGTATCAAA80.1080935008782597No Hit
CGGAAGGATCATTACCACACCTGAAAACTTTCCACGTGAACCGTATCAAA80.1080935008782597No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATTAAA80.1080935008782597No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACATGAACCGTATCAAA80.1080935008782597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)