FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH601.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH601.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5549
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC307255.36132636511083No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT141725.536132636511084No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA951.712020183816904No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT340.6127230131555236No Hit
CGGAAGGATCATTACCACACCTAAAAACTATCCACGTGAACCACTTTGTA320.5766804829699045No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC280.5045954225986664No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT280.5045954225986664No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATGAAC200.36042530185619026No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCGTTAACCAA180.3243827716705713No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC170.3063615065777618No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC160.28834024148495224No Hit
CGGAAGGATCATTACCACACCTAAAAAACTATCCACGTGAACCGTTAAGC160.28834024148495224No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGC130.2342764462065237No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAC130.2342764462065237No Hit
CGGAAGGATCATTACCACACCTAAAAACCTTCCACGTGAACCGTATCAAC120.21625518111371417No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA110.19823391602090465No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT110.19823391602090465No Hit
CGGAAGGATCATTACCACACCTAAAAACTCTCCACGTGAACCGTATCAAC100.18021265092809513No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAC100.18021265092809513No Hit
CGGAAGGATCATTACCACACCTAAAGACTTTCCACGTGAACCGTATCAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATTAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAGAAACTTTCCACGTGAACCGTATCAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGGACCGTATCAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTGTCAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTACCAAC80.14417012074247612No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCATGTGAACCGTATCAAC70.1261488556496666No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT70.1261488556496666No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCTACGTGAACCGTATCAAC70.1261488556496666No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT60.10812759055685708No Hit
CGGAAGGATCATTACCACACCTAAAACTTTCCACGTGAACCGTATCAACA60.10812759055685708No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT60.10812759055685708No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT60.10812759055685708No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGCGAACCGTATCAAC60.10812759055685708No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGCATCAAC60.10812759055685708No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT60.10812759055685708No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTAAACCGTATCAAC60.10812759055685708No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCCCGTGAACCACTTTGTAT60.10812759055685708No Hit
CGGAAGGATCATTACCACACCTAAAAACTTCCCACGTGAACCGTATCAAC60.10812759055685708No Hit
CGGAAGGATCTTACCACACCTAAAAACTTTCCACGTGAACCGTATCAACA60.10812759055685708No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATAAAC60.10812759055685708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)