FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8056
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT704087.38828202581927No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC2272.8177755710029793No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT921.1420059582919564No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT260.3227408142999007No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT210.26067527308838134No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA200.24826216484607747No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA180.22343594836146974No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT170.21102284011916583No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT170.21102284011916583No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT140.1737835153922542No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT140.1737835153922542No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT130.16137040714995035No Hit
CGGAAGGATCATACCACACCAAAAACTATCCACGTGAACCACTTTGTATT120.14895729890764647No Hit
CGGAAGGATCATTACCACACCAAAAACTATTCACGTGAACCACTTTGTAT110.1365441906653426No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT100.12413108242303873No Hit
CGGAAGGATCATTATCACACCAAAAACTATCCACGTGAACCACTTTGTAT100.12413108242303873No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT90.11171797418073487No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT90.11171797418073487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)