FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27330
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT1869368.397365532382No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC368113.468715697036224No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCCCTTTGTAT3221.1781924624954263No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGTATCAACA2070.7574094401756312No Hit
CGGAAGGATCATTACCACACCAAAAACTATCAACGTGAACCACTTTGTAT1270.46469081595316497No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCACTTTGTA900.3293084522502744No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTTAACCACTTTGTAT830.3036955726308086No Hit
CGGAAGGATTATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT730.2671057446030004No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTATGTAT610.22319795096963047No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGGACCACTTTGTAT580.21222100256128798No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCGCTTTGTAT560.20490303695572631No Hit
CGGAAGGATCATTACCACACCTAAAACTATCCACGTGAACCACTTTGTAT550.2012440541529455No Hit
CGGAAGGATCATTACCACACCAAAAACTTTCCACGTGAACCGTATCAACA510.18660812294182216No Hit
CGGAAGGATCATTACCACGCCAAAAACTATCCACGTGAACCACTTTGTAT500.18294914013904134No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGCGAACCACTTTGTAT450.1646542261251372No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCCCGTGAACCACTTTGTAT440.16099524332235637No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAGCCACTTTGTAT440.16099524332235637No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC430.15733626051957555No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAAACACTTTGTAT430.15733626051957555No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCAATTTGTAT430.15733626051957555No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACCTTGTAT420.15367727771679474No Hit
CGGAAGGATCATTACAACACCAAAAACTATCCACGTGAACCACTTTGTAT410.1500182949140139No Hit
CGGAAGGATCATACCACACCAAAAACTATCCACGTGAACCACTTTGTATT400.14635931211123307No Hit
CGGAAGGATCATTACCACACCAAAAACTACCCACGTGAACCACTTTGTAT380.13904134650567143No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTAAACCACTTTGTAT380.13904134650567143No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCAAGTGAACCACTTTGTAT380.13904134650567143No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCGCGTGAACCACTTTGTAT360.13172338090010977No Hit
CGGAAGGATCATTACCAAACCAAAAACTATCCACGTGAACCACTTTGTAT330.1207464324917673No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTCTGTAT330.1207464324917673No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAC300.10976948408342481No Hit
AGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT300.10976948408342481No Hit
CGGAAGGATCATTACCACACCAAAAACTATTCACGTGAACCACTTTGTAT290.10611050128064399No Hit
CGGAAGGATCATTACCACACCAAAAACTATCTACGTGAACCACTTTGTAT280.10245151847786316No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGCAT280.10245151847786316No Hit
CGGAAGGATCATTACCACACCGAAAACTATCCACGTGAACCACTTTGTAT280.10245151847786316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)