FastQCFastQC Report
Fri 3 May 2024
000000000-L7NBT_l01_n01_repeat-ITS-OOATH575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L7NBT_l01_n01_repeat-ITS-OOATH575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC1517189.05259450575252No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCAACGTGAACCGTATCAAC1791.0507161305470767No Hit
CGGAAGGATCATTACCACACCAAAAACTATCCACGTGAACCACTTTGTAT680.39915473115754874No Hit
CGGAAGGATTATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC620.363935196055412No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCCTATCAAC490.287626203334116No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGCGAACCGTATCAAC350.20544728809579715No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAGCCGTATCAAC350.20544728809579715No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAA330.19370744306175158No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTAAACCGTATCAAC310.181967598027706No Hit
CGGAAGGATCATTACCACGCCTAAAAACTTTCCACGTGAACCGTATCAAC300.17609767551068325No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGGACCGTATCAAC300.17609767551068325No Hit
CGGAAGGATCATACCACACCTAAAAACTTTCCACGTGAACCGTATCAACC290.1702277529936605No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACATGAACCGTATCAAC290.1702277529936605No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCGACGTGAACCGTATCAAC250.14674806292556938No Hit
TGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAAC250.14674806292556938No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTACACGTGAACCGTATCAAC240.1408781404085466No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCAGC230.13500821789152384No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCTACGTGAACCGTATCAAC230.13500821789152384No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATCGAC230.13500821789152384No Hit
CGGAAGGATCATTACCACACCTAAAACCTTTCCACGTGAACCGTATCAAC220.12913829537450106No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCGCGTGAACCGTATCAAC220.12913829537450106No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATAAAC210.12326837285747828No Hit
CGGAAGGATCATTACCACACCTAAAAACTTTCCACGTGAACCGTATTAAC180.10565860530640996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)