FastQCFastQC Report
Fri 14 Jul 2023
000000000-L66WL_l01.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L66WL_l01.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5920469
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA139807123.614193402583478No Hit
GTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTAGAG79787613.47656748139379No Hit
GTACGTCATCCCCACCTTCCTCTCTACTTGCGTAGGCAGTCTCACTAGAG3656476.175980315073012No Hit
AAGGGTTCAGCTTTGTCAACAATGGAGGCAACAACTTTGCTGTGAACATC2402224.057482608219045No Hit
GTACGTCATCCCCACCTTCCTCCGGCTTATCACCGGCAGTCTGTTCAGGG672501.1358897411674649No Hit
GTACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCATTAGAG365860.6179578003026449No Hit
GTACGTCATCCCCACCTTCCTCACAGTTTGCACTGGCAGTCTTGTTAGAG341800.5773191279271963No Hit
GTACGTCATCCCCACCTTCCTCCGGCTTGTCACCGGCAGTCTCCTTAGAG169090.2856023737308649No Hit
GGTATCTAATCCTGTTACTCCTGTCTCTTATACACATCTGACGCTGCCGA84930.1434514731856547No Hit
GGTATCTAATCCGGTTACTCCTGTCTCTTATACACATCTGACGCTGCCGA66770.11277822753569017No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA59640.10073526269624923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)