FastQCFastQC Report
Fri 14 Jul 2023
000000000-L66WL_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L66WL_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5920469
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC97218716.420776799946086Illumina Single End PCR Primer 1 (100% over 8bp)
ACGCGTGA5174858.740608218706997No Hit
TCTACACT5138198.67868744857882Illumina Single End PCR Primer 1 (100% over 8bp)
GATAGCGT4596307.763405230227538No Hit
CGATCTAC4033086.812095460680564No Hit
CTAGTATG3451205.8292679177950255No Hit
CTAGAGCT2612014.411829535801978No Hit
GTCTAGTG2391634.03959551177449No Hit
AAGCAGCA2386274.030542174952694No Hit
TAGTGTAG2295843.8778008971924347No Hit
CGTCGCTA2208503.730278800547727No Hit
GCTCTAGT1417122.3935941561386436No Hit
TGCGTACG1128351.9058456348644No Hit
CTACTATA991311.6743774859728173No Hit
TGCGTCAC941521.5902794187419949No Hit
TCGACGAG795631.3438631297621861No Hit
GTCAGATA768161.297464778550483No Hit
GATCGTGT764621.2914855225151927No Hit
CGTTACTA763581.289728904922904No Hit
ATATACAC666911.126447921608913No Hit
ACGTCTCG565250.9547385519626908No Hit
TACGAGAC530150.8954527082229465TruSeq Adapter, Index 14 (100% over 8bp)
AGAGTCAC507390.8570098078378589No Hit
GACACTGA453260.7655812402699854No Hit
ACGACGTG406540.6866685730471691No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)