FastQCFastQC Report
Fri 14 Jul 2023
000000000-L66WL_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L66WL_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5920469
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTACG2461164.157035532151253No Hit
CGAGAGTT2420484.08832475940673TruSeq Adapter, Index 13 (100% over 8bp)
TAGTCTCC2078823.5112420992323408No Hit
GATCTACG2005123.38675871793265No Hit
CGAGCGAC1980573.3452924084223734No Hit
GTAACGAG1946543.287813853936234No Hit
TAGCAGCT1925653.252529487106511No Hit
CTCGACTT1776153.0000157082150083No Hit
CGAAGTAT1740212.939311057958415No Hit
ACTACGAC1727912.9185356768188466No Hit
ATAGTACC1676872.8323262903665234No Hit
ACTCACTG1565802.6447229096208424No Hit
AGTCGCAG1534742.592260849604989No Hit
CGCGATAT1445872.4421544982331636No Hit
CTGCGTAG1402012.3680725293891416No Hit
TCTCTATG1359342.296000536443988ABI Solid3 Adapter B (100% over 8bp)
ACGTGCGC1350312.280748366387866No Hit
GACATAGT1277632.1579878215729194No Hit
TGCGTCAA1239472.093533468378941No Hit
TACTAGGT1227522.0733492566213925No Hit
CTACGACC1090931.8426411826495501No Hit
GTCTGCTA1082891.8290611774168568No Hit
TAACGTCC1009031.7043075472568134No Hit
GAGACTTA1007561.7018246358523286No Hit
GCGATACG938941.5859216558688172No Hit
GTTACAGC930791.5721558545446317No Hit
GCGTATAC923311.5595217203231704No Hit
ACGCTACT905961.5302166095287384No Hit
ACGTACGT832401.4059696959818555No Hit
AACGCTGA717661.212167482001848No Hit
TGCTCGTA573650.9689266171311766No Hit
CTATCGTG507410.8570435889454028No Hit
CGTAGCGA483580.8167933993067102No Hit
ACTGTGTA385660.6514010967712186No Hit
GTCTATGA314650.5314612744361975No Hit
TATAGCGA239620.40473144948482964No Hit
CTTGTAAT179980.3039961867885804Illumina PCR Primer Index 12 (100% over 8bp)
GCCAATAT158610.26790107337780167Illumina PCR Primer Index 6 (100% over 8bp)
ATAGCGCT69370.11716977151641195No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)