FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_groPIELPathoPool4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_groPIELPathoPool4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG9939.6No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT4718.8No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG52.0No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG41.6No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG31.2No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT20.8No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG20.8No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGTATGATACGTGGCATTT20.8No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.8No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.8No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT20.8No Hit
GAGGAGGGCATCGTCCCAGGCGGCGGTACTGCGCTTCTGCGCTCTTCCAC10.4No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAAGCGACTCGCGGCAAAT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCTAATTCAGTGCCATCCCCCCTAA10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGCGACGTTTTCGTTTGGGAGTCTG10.4No Hit
TACCCTTACGATGTTCCTGATGATGCCCAAGCTGAAACCTTCTGGCTCTT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTCTCGAAGATGTCGGTGGGT10.4No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTAGGGTGCGGGGAGGGACCCG10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTTACTCG10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGGATGGAGCAGGGATTGACTCG10.4No Hit
CTGTAAAGATAAAAGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
CTGTAAAGATAAATGGGGCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
CTGTAAAGATAAATGGGTCATCACACTCGAAGATTTCGTTGGGGACTCTG10.4No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT10.4No Hit
CTGTAAAGATAAAAGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA10.4No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTTCATGATACGTGGCATTT10.4No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCAAAGATTTCGTTGGGGACTCTG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGG10.4No Hit
TACCCTTACGCTGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC10.4No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAATTTCCGATCGTGACGTTCGT10.4No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACTTTCGT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTTAACTCTGTGCTAACGGGCCGCT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG10.4No Hit
CTGTAAAGATAAATGGGTCATCTAATCTGGTTGACAAAGCTGAAGACTTC10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCGAGGGCTCTAACAGTCCGTCGGA10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT10.4No Hit
CGGTTCATCTGCTTTTGTCATCGCACGATTATGTCGCTTGTCTTGACTCT10.4No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTTG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGGTCTAACAGTCCGTCGGA10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG10.4No Hit
CTGTAAAGATAAATGGGTCATCTAATGAGTATGGCGCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCGTTGCTCTAAGATTTCGTT10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCGGGGATTGACTCG10.4No Hit
CTGTAACGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCCCGTGTTCTCGAAGCTTTCGCTG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGTTCGTTGAGGACTCTG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGCCTCT10.4No Hit
TACCCTTACGATGTTCCTGGTACGCGCTCGTATATTTCTTTGGGGCTTAT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTTAGGCGCGAAGATGTCGGTGGGT10.4No Hit
GATGAGTCCGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT10.4No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATCCGATTCGTGTTAAAT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGTCTCGAAGCTTTCGTTG10.4No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTTGCCCGAAAATATCGCTGT10.4No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGCGCTCAAATAGTTCGTTGGT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGTAGGCTCGAAGATTTCGTTGGGA10.4No Hit
GAAGCAGACATCGTCCCAGGCGGCGGTCCTGCTCTTCTGCTCTCTTCCAC10.4No Hit
GATGAGTCAGCGTCGAACTGGAGAACGTCACCACCGACATGCGCCGCAGC10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGGGCTCGAAGATTTCGTTGGG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG10.4No Hit
GATGAGTCAGCGTCGAACAGTGGCACTTGCGCAGCCGCAGGCGGGCCGAT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCGTTACGAGTTCGGGGGGGACCTTG10.4No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT10.4No Hit
TACCCTTACGATGTTCCTGTTACGCTTATCGTCTTTTTAAAGGAAGGCGT10.4No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACCTTCGT10.4No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGATCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCTAATATTTCTTTGGG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG10.4No Hit
TACCCTTACGATGTTCCTGATTACGTTGTGGACTCTGCTCTTACTCGAAT10.4No Hit
CTGTAAGGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTTCCAGCCCGCCTAA10.4No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.4No Hit
CTGTAAAGGTAAACGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGATCGTTTGGGACTCTT10.4No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTAACCTTCGT10.4No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGTTTTCGTATGTGGCTCTT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG10.4No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAACATTTCGTGGGGAC10.4No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCGGGGATTGACTCG10.4No Hit
TACCCTTACGGTGTTCCTGATTACGCCTTCGTTCGCTCTAAGATTTCGTT10.4No Hit
TACCCTTACGATGTTCCTGATTACGCATCATGTGCCTCCAGCACGTCGCT10.4No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)