FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_groPIELPathoPool2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_groPIELPathoPool2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG34059.23344947735192No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT539.233449477351916No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG162.7874564459930316No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG91.5679442508710801No Hit
CTGTAAAGATAAATGGGGCATCTAAAGAGTATGGAGCTGGGATTGACTCG30.5226480836236934No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG30.5226480836236934No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.5226480836236934No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGGATGGAGCTGGGATTGACTCG20.34843205574912894No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.34843205574912894No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.34843205574912894No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT20.34843205574912894No Hit
GATGAGTCAGCGTCGAACATACAACATTTATTGCATGATACGTGGCATTT20.34843205574912894No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT20.34843205574912894No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGTAGATTTCGCTTGG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCTCGATCGTATCTTTCGTTA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCGATGTGTTAGTTGGGGGCCCTT10.17421602787456447No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGCTCGTGACGTTCGT10.17421602787456447No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATGGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCTCAGATTGCGTTGGGGA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGCTTTCGTTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCGTTAGGGCTAAGAGTTTGTCTCT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATATAGCCCAACGATTTGGTTCGTGGCAATT10.17421602787456447No Hit
CGGTTCGTCCGCTTCTGCTATGCGTTAGATATCGCGCTTGTGATCCTCTT10.17421602787456447No Hit
TACCCTTACTATGTTCCTGATTACGCTCGATTTGCTGGTAGCCGGCCTCT10.17421602787456447No Hit
CTGTAACGATAAATGGGTCATCTAAACAGGATGGAGATGGTATAGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGAGCTAAGATTGCGTGGGTGA10.17421602787456447No Hit
CGGTTCGTACGCTTCTGCTATGCGTTCCTTATTTCGCTCGTCAGTAGCGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTACAATTCCTTGGGGCCTCT10.17421602787456447No Hit
CTGAAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTAATTCATTGCCAGCGCGCCGAA10.17421602787456447No Hit
TACCCTTACGCTGTTCCTGCTTACGCTGGCTCTAGTATTTCGTGTGGGAC10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAGGTGTTAGTTGGGGGCCTTT10.17421602787456447No Hit
TACCCTTACTATGTTCCTGATTACGCGCGGGGTTATCTTATCGTTTTGGG10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAATTCTTCTGTCTCTGATATTCTCTCT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGTG10.17421602787456447No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGACATCCCGATTGTGACCCTCGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG10.17421602787456447No Hit
TACCTTCCGATGTTCCTGATTACGCCTTTGGCTCGAAGATTTCGTTGGGT10.17421602787456447No Hit
CTGTAAAGATAAAAGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTTTCTCGAATGTCCGCCTGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGGAGCTCGAAAATTTCGCCGGGGTCT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAGGGTTTCGTTGGGGGCCTTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTTTTCGAAGCGTTCGGTG10.17421602787456447No Hit
CTGTAAAGATAAATTGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGGTCGAAGATTTCGTGGGGGA10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATCTGAGTGTTCCAGGACGTCTTT10.17421602787456447No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAAGATTTCGTTGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGTTCGCAGCGCGCCTAA10.17421602787456447No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATAATCCAGTGCCATCCCTCCTAA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAATCTATCGTTGCGTTCTCTG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCACCACCCCCACCATGACCAAGC10.17421602787456447No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
CTGTAAAGGTAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
CTGTAAAGATAAATGTGTCATCTCAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCGAGATTGAATCTTTCGTTT10.17421602787456447No Hit
GATGAGTCCGCGTCGAACATGCAACCTTTTTTGCATGATACGTTGCCTTT10.17421602787456447No Hit
TACCCTTACGCTGTTCCTGCGTACGCTGGGCGCTAAGATTTCGTTGGGGA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGGACAGTGCGCAGACTTCGCGGCTAA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATAACGTCTAGTCAAGCGACTCGCGGTCAAT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGCTCTGTT10.17421602787456447No Hit
TACCCTTACGATGCTCCTGATTACGCAGTGTGCTCGAACAGTCCGCCTGG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATAGGTCCGAAGCTGCCGGTCTAA10.17421602787456447No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGCTGTTCCTGAGTACGCCGAGGGCTGTAATATTACGTTGGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCGGTGATCGCACATTGAGTTGGGGA10.17421602787456447No Hit
TACCCTTACGCTGTTCCTCCTTACGCCTCGCATATTTCTTTGGGTCCTAT10.17421602787456447No Hit
GAGGGGGTCATCGTCCCCGGCGGCGGCATCGCCCTGCTGAAGGCGACCAA10.17421602787456447No Hit
TACCCTTACGGTGTTCCTGATTACGCTTTAAGATTTCGTTTGGGACTCTT10.17421602787456447No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATCCCGATCGTCACCTTCGT10.17421602787456447No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATCT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTACGGGATCGAATATTTCGTG10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTAGGGAGCTGGGATTGACTCG10.17421602787456447No Hit
CTGTAAAGATAAACGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGCTGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT10.17421602787456447No Hit
CGGTTCGTGCGCTTCTGCGAGGCGTTCGATATTCCGATCGTCACCTTCGT10.17421602787456447No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
GAAGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT10.17421602787456447No Hit
CTGGAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGATCACGTAGCCGGCTTCA10.17421602787456447No Hit
TACCCTTACTATGTTCCTGATTACGCGTATGTGGTTCCCAGCCCGCCTAA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTGCCCAAAGTTCGGCTGA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGGTCCCGGTCTCGAATATGGCGCTGT10.17421602787456447No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTTCATGATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCTAATTCGCAGACATCGCGCCTAA10.17421602787456447No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGACAGCTTGCCTGG10.17421602787456447No Hit
GAGGAGGGCATCGTCCCCGGCGGCGGCACCGCCCTACTCCGCGCCCGCGA10.17421602787456447No Hit
GAGGAGGGAATCGTTCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGTTTCGGCTCTTTGATTTGGGAG10.17421602787456447No Hit
TACCCTTACGGTGTTCCTGATTACGCTTCCTGATCTCGAGTCGGGCGCTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCTTGCTCTCAGATTGCGAGGCGGA10.17421602787456447No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATCCCGATCGTGACTTTCGT10.17421602787456447No Hit
GAGGAGGTCATCGTCCCCGGCGGCGGCGTCGCGCTGCTGCGCGCTTCGCA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA10.17421602787456447No Hit
TACCCTTACTATGTTCCTGATTACGCTTGTGCTCAAGTAATAGGACAATG10.17421602787456447No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGATATCCCGCTCGTGACGTTCGT10.17421602787456447No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
CTGTAAAGATAATTGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGGCGTTTTCGATGCTG10.17421602787456447No Hit
CTGTAAAAATAAATGGGTCATCTAAAGAGTATGGAGCTGGGTTTGACTCG10.17421602787456447No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG10.17421602787456447No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACTCATATGTAAACTGCTGCCCGCCTCA10.17421602787456447No Hit
CTGTAAAGATAAATGGTTCCTAGAACGCTTCTGTCGGTGGTCTTGCAATG10.17421602787456447No Hit
TACCCTTACAATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCTCTCAGATTTCGCGGGGGA10.17421602787456447No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACGTTCGT10.17421602787456447No Hit
GAAGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA10.17421602787456447No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCCTTT10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGAGTGACTCG10.17421602787456447No Hit
GATGAGTCAGCGTCGAACATGCTACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGCTGTTCCTGATTACGCATATTGTGCACACAGCCCTCCTAA10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGTGTTCGAGTTTTTCGTTG10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGACTTACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGGCTCTGATATTTCGTTTGGGAC10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGTGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG10.17421602787456447No Hit
GAGGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACGCG10.17421602787456447No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACTTTCGT10.17421602787456447No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCGCTACGATTTAGTGTGGGACTTTG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGGGGTAGAATATTTCGTTGGG10.17421602787456447No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCCTGATACGTGGCATCT10.17421602787456447No Hit
GATGGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGGTGTTCCTGATTACGGTCTCTGGTCTCGAATCTTTCGTTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGGTGAGTTCGCAGATTTCGCGGGGGA10.17421602787456447No Hit
GATGAGTCAGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
GATAAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT10.17421602787456447No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT10.17421602787456447No Hit
CTGTAAAGCTAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGGTCTTTGACTTCGCGCGGGA10.17421602787456447No Hit
CTGTAAAGCTAAATGGTTCATCTAAAGAGTATGGAGCTGGGATTGCCTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGAGTTCGTTGGGGACTCTT10.17421602787456447No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGCAGCTGGGATTGACTCG10.17421602787456447No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGCCCAGTGATTTTATAG10.17421602787456447No Hit

[FAIL]Adapter Content

Adapter graph