FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA2039.89278752436647No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG1426.920077972709551No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG914.434697855750487No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT763.7037037037037033No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG713.4600389863547756No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG612.9727095516569197No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG572.7777777777777777No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG532.582846003898635No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT502.4366471734892787No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG452.1929824561403506No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG351.705653021442495No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG331.608187134502924No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG321.5594541910331383No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG311.5107212475633527No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGATTTCGTTG311.5107212475633527No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG311.5107212475633527No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT301.461988304093567No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG301.461988304093567No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG291.4132553606237817No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC160.7797270955165692No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG140.682261208576998No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA140.682261208576998No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT120.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG110.5360623781676412No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA100.4873294346978557No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG90.43859649122807015No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC80.3898635477582846No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG70.341130604288499No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG60.29239766081871343No Hit
TACCCTTACGATGTTCCTGATTATGGTCTGTGGGCTCGAAGATTTCGTTG60.29239766081871343No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG50.24366471734892786No Hit
TACCCTTACGATGTTCCTGATTACGATCTCGGGCTCGAAGATTTCGTTGG50.24366471734892786No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG50.24366471734892786No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG50.24366471734892786No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGG50.24366471734892786No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT50.24366471734892786No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGAGGCTCGAAGATTTCGTTGG40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGCTTTCGTTG40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG40.1949317738791423No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGGTGG30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCGCGGGCTCGAAGATTTCGTTGGGG30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGAATCTG30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGCTGG30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGTCTCGAAGATTTCGTTGG30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGGTTTCGTTGG30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA30.14619883040935672No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG30.14619883040935672No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG30.14619883040935672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)