FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA28013.326987148976677No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA21510.233222275107092No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1326.282722513089005No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1085.140409328891004No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA1085.140409328891004No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA1004.75963826749167No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT582.760590195145169No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG341.618277010947168No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG331.5706806282722512No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA281.332698714897668No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG231.0947168015230844No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT160.7615421227986673No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG160.7615421227986673No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT140.666349357448834No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG140.666349357448834No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA120.5711565920990005No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG120.5711565920990005No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG110.5235602094240838No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG90.42836744407425037No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA90.42836744407425037No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA80.38077106139933364No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA70.333174678724417No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA70.333174678724417No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA70.333174678724417No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA70.333174678724417No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.333174678724417No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG70.333174678724417No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG70.333174678724417No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG60.28557829604950025No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG60.28557829604950025No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA60.28557829604950025No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG50.23798191337458352No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG50.23798191337458352No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA50.23798191337458352No Hit
TACCCTTACTATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA50.23798191337458352No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTGGGCTCGAAGATTTCGTTGG40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG40.19038553069966682No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA40.19038553069966682No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTTCTGCCATCCCTCCTAA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTTCCAGCCCGCCTAA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTCA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCATAA30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGTCCGGGGCTCGAAGATTTCGTTGGG30.14278914802475012No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAC30.14278914802475012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)