FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA806.739679865206402No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG705.8972198820556025No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT655.475989890480202No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG302.527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG282.358887952822241No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG231.9376579612468408No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG221.8534119629317607No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA211.7691659646166806No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG201.6849199663016006No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT201.6849199663016006No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA201.6849199663016006No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA151.2636899747262005No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG151.2636899747262005No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG151.2636899747262005No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG141.1794439764111204No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG131.0951979780960404No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG110.9267059814658803No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG100.8424599831508003No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC90.7582139848357203No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG90.7582139848357203No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG90.7582139848357203No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG80.6739679865206403No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA80.6739679865206403No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT80.6739679865206403No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA70.5897219882055602No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.5897219882055602No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.5897219882055602No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG60.5054759898904801No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT60.5054759898904801No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG60.5054759898904801No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT60.5054759898904801No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG60.5054759898904801No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA50.42122999157540014No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGT50.42122999157540014No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT50.42122999157540014No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT50.42122999157540014No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG40.33698399326032014No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.33698399326032014No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTTGGCTCGAAGATTTCGTTG30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGA30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG30.2527379949452401No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA30.2527379949452401No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTGGG20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.16849199663016007No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGTGGGCTCGAAGATTTCGTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGCGCTCGAAGATTTCGTTG20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTAAGATTTCGTTGGGGA20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGGTCTCGAAGATTTCGTTGGGGA20.16849199663016007No Hit
GATAAGTCAGCGTCGAACATGCAACATTTATTGCATAATACGTGGCATTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGCTTTCGTTG20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAGATTTCGTTGGGGACTCTGT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT20.16849199663016007No Hit
GATGCGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAATATTTCGTTGGGGACTCT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGTGCTCGAAGATTTCGTTG20.16849199663016007No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTGTCTCTACTAGTTCGTGTGGGAC20.16849199663016007No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT20.16849199663016007No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG20.16849199663016007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)