FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA21312.536786344908771No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1639.59387875220718No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1398.181283107710417No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA905.29723366686286No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT603.531489111241907No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG422.4720423778693346No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT342.0011771630370805No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG311.8246027074749853No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG301.7657445556209534No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA301.7657445556209534No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT231.353737492642731No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG221.2948793407886992No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT191.1183048852266038No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA160.9417304296645085No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG160.9417304296645085No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG130.7651559741024131No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG120.7062978222483813No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA120.7062978222483813No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA110.6474396703943496No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG110.6474396703943496No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA100.5885815185403178No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA100.5885815185403178No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG80.47086521483225424No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG70.4120070629782225No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG70.4120070629782225No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG70.4120070629782225No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA60.35314891112419067No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG60.35314891112419067No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG60.35314891112419067No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA60.35314891112419067No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA50.2942907592701589No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG50.2942907592701589No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA50.2942907592701589No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG50.2942907592701589No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG40.23543260741612712No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT40.23543260741612712No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA40.23543260741612712No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA40.23543260741612712No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT40.23543260741612712No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT40.23543260741612712No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG40.23543260741612712No Hit
TACCCTTACGATGTTCCTGCTTACGCATAGTGCAAATGCAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGGTTGCCAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGAGCAAACAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGCTGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA30.17657445556209533No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGTAACAGCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCATCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTCA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTGCTCGAAGATTTCGTTGGGGACTCTGTC20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGAGTTCGTT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTTCGTTGGGGACTCT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAGGATTTCGTTGGGGA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCCTAAGAGTTTGCCAGCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAATATTTCGTTGG20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCGCCTCGGGCTCGAAGCTTTCGTTG20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATCATGTGCAAACAGCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.11771630370806356No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCATCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAACCCGCCTAA20.11771630370806356No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACTCCGTGGGCTCGAAGATTTCGTTGGG20.11771630370806356No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGACTAA20.11771630370806356No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTT20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCTCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCCGCCCGCCTAA20.11771630370806356No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA20.11771630370806356No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.11771630370806356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)