FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20110.79484425349087No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG975.209452201933405No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT774.135338345864661No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT683.6519871106337276No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG402.1482277121374866No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG392.0945220193340495No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG301.611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG291.5574650912996777No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG201.0741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA180.966702470461869No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA180.966702470461869No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT160.8592910848549946No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA150.8055853920515575No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA140.7518796992481203No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG130.6981740064446832No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA120.644468313641246No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG110.5907626208378088No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG110.5907626208378088No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG90.4833512352309345No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT90.4833512352309345No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA80.4296455424274973No Hit
TACCCTTACGATGTTCCTGATTACCGTCCGTGGCTCGAAGATTTCGTTGG80.4296455424274973No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT80.4296455424274973No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT80.4296455424274973No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA80.4296455424274973No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG70.37593984962406013No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA70.37593984962406013No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT70.37593984962406013No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA60.322234156820623No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA60.322234156820623No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT60.322234156820623No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA60.322234156820623No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA50.26852846401718583No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT50.26852846401718583No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT50.26852846401718583No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA50.26852846401718583No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG50.26852846401718583No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG50.26852846401718583No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA40.21482277121374865No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT40.21482277121374865No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT40.21482277121374865No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT40.21482277121374865No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.21482277121374865No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT40.21482277121374865No Hit
TACCCTTACGATGTTCCTGATTACGAGTCTGTGGCTCGAAGATTTCGTTG40.21482277121374865No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.1611170784103115No Hit
GAAGAGGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGCTTTCGTTG30.1611170784103115No Hit
GAGGAAGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGGTGGGGA30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTT30.1611170784103115No Hit
ACACCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGCTCGAAGATTTCGTTG30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGATGGGGA30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGATCGAAGATTTCGTTGGGGACTCTG30.1611170784103115No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT30.1611170784103115No Hit
TACCCTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTGT30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCGCGAAGATTTCGTTGGGGACTCTG30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.1611170784103115No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT30.1611170784103115No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGTAACAGCCCGCCTAA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG20.10741138560687433No Hit
GAGGAGGGCATCGTCCCCGGTGGCGGTACGGCGCTGCTGTACGCGACCAA20.10741138560687433No Hit
ACGCCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT20.10741138560687433No Hit
GAGGAGGGAATCGTTCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGGTTGGGA20.10741138560687433No Hit
ACGCCCAAGCAGATTTACAACCGCATGCAGAACGTCTATGCCGACCAGCA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT20.10741138560687433No Hit
TACCCTTACGCTGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATGTCT20.10741138560687433No Hit
TACCCTTACGCTGTTCCTGACTACGCTCTACGATTTAGTTTGGGACTCTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTCTCGAATCTTTCGTTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTGGGGAC20.10741138560687433No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTGCAAACAGCCCGCCTAA20.10741138560687433No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCCACGATTTCGTTTGGGACTCTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGTCTCGAAGATTTCGTTGGG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGCAGATTTCGTTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGGTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTGCTCGAAACTTTCGTTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG20.10741138560687433No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCGCTCAGATTTCGGTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT20.10741138560687433No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCGGGGCTCGAAGATTTCGTTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATAACGTCTAGCCATCCGATTCGTGTTAAAT20.10741138560687433No Hit
GAGGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCCCTCAGATTTCGCTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGGAGATTTCGTTGGGGA20.10741138560687433No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGCTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGGTTTGTGTTCTCGAATCTTTCGTTT20.10741138560687433No Hit
ACGCCCAAGCAGATTTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTTCTCGAATCTTTCGTTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGCAGATTTCG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGCTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTTGTTCTCGAATCTTTCGTTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAGGATTTCGTTGGGGACTCTG20.10741138560687433No Hit
GAAGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.10741138560687433No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.10741138560687433No Hit
TACCCTTACGCTGTTCCTGCGTACGCTGTGCCCTCAGATTTCGGTGGGGA20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATCCGATTCGTGTTAAAT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGCTTACGCTCTACGATTTCGTTTGGGACTCTT20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGTGGCTCGAAGATTTCGTTG20.10741138560687433No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAAGATTTCTTTGGT20.10741138560687433No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)