FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA1039.363636363636365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT847.636363636363637No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA807.2727272727272725No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA666.0No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA635.7272727272727275No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG524.7272727272727275No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA363.272727272727273No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT282.5454545454545454No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA232.090909090909091No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA181.6363636363636365No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG181.6363636363636365No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG171.5454545454545454No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT171.5454545454545454No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG161.4545454545454546No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG131.1818181818181819No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG131.1818181818181819No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG121.090909090909091No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT121.090909090909091No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG111.0No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG90.8181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG90.8181818181818182No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC90.8181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG90.8181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC80.7272727272727273No Hit
TACCCTTACGATGTTCCTGATCAGTGGTACTCGAAGATTTCGTTGGGGAC50.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA50.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG50.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.36363636363636365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT40.36363636363636365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT40.36363636363636365No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG30.27272727272727276No Hit
TACCCTTACGATGTTCCTGATTACGAAGATTTCGTTGGGGACTCTGTCTC30.27272727272727276No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT30.27272727272727276No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.27272727272727276No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGAAGATTTCGTTGGGGACTCT30.27272727272727276No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA30.27272727272727276No Hit
GATGCGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.18181818181818182No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGGCTCGAAGATTTCGTTGGG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGG20.18181818181818182No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA20.18181818181818182No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.18181818181818182No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGCTTTCGTTG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.18181818181818182No Hit
CTGTAAAGATAAACGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCACCTAA20.18181818181818182No Hit
GATGAGTCAGCGTCGAATAGCCGTTCACTATGCCAAGCGTCAGCAGGGCC20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.18181818181818182No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.18181818181818182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)