FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA119745.98540145985402No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA833.1886285055704953No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT742.8428736073761045No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA622.3818670764502494No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA602.305032654629274No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA582.228198232808298No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA501.920860545524395No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA481.844026123703419No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG481.844026123703419No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG260.9988474836726854No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA240.9220130618517095No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA200.768344218209758No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA180.6915097963887822No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG180.6915097963887822No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA160.6146753745678064No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA140.5378409527468306No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT130.4994237418363427No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA130.4994237418363427No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA120.46100653092585475No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG110.42258932001536686No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG110.42258932001536686No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG110.42258932001536686No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA100.384172109104879No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA90.3457548981943911No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC90.3457548981943911No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT90.3457548981943911No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA90.3457548981943911No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG80.3073376872839032No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTTGCAAACAGCCCGCCTAA70.2689204763734153No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGGGCAAACAGCCCGCCTAA60.23050326546292738No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT60.23050326546292738No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT60.23050326546292738No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC60.23050326546292738No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG60.23050326546292738No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA60.23050326546292738No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT50.1920860545524395No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCATAA50.1920860545524395No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG50.1920860545524395No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.1920860545524395No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT40.1536688436419516No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTGCAAACAGCCCGCCTAA40.1536688436419516No Hit
TACCCTTACGTTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA40.1536688436419516No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT30.11525163273146369No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.11525163273146369No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.11525163273146369No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA30.11525163273146369No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA30.11525163273146369No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.11525163273146369No Hit
TACCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT30.11525163273146369No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA30.11525163273146369No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.11525163273146369No Hit
TACCCTTAGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT30.11525163273146369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)