FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA17414.859094790777114No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG14011.955593509820666No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT363.0742954739538857No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG292.4765157984628523No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG272.305721605465414No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT262.220324508966695No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG252.134927412467976No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG242.0495303159692573No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG231.964133219470538No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG201.7079419299743808No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG191.6225448334756618No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC181.5371477369769428No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGGCTCGAAGATTTCGTTGGGG171.4517506404782237No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT151.2809564474807857No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG151.2809564474807857No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA141.1955593509820666No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG141.1955593509820666No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA121.0247651579846286No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG121.0247651579846286No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG110.9393680614859093No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC100.8539709649871904No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG80.6831767719897524No Hit
TACCCTTACGATGTTCCTGATACGCGGGCTCGAAGATTTCGTTGGGGACT70.5977796754910333No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT70.5977796754910333No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG70.5977796754910333No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG60.5123825789923143No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG60.5123825789923143No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA60.5123825789923143No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG60.5123825789923143No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT50.4269854824935952No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG50.4269854824935952No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG50.4269854824935952No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGAAGATTTCGTTGGGGACTCT40.3415883859948762No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT40.3415883859948762No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.3415883859948762No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGACTCTGT30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGCTTTCGTTG30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTGGGGAC30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGT30.25619128949615716No Hit
TACCCTTACGATGTTCCTGATTACGCTTGACGATTTCGTTGGGGACTCTG20.1707941929974381No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.1707941929974381No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGTGCTCGAAGCTTTCGTTG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGGTTTCGTTGG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGGCTCGAAGATTTCGTTG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCTGCTCGAAGATTTCGTTGTGGACT20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGAGCTCGAAGATTTCGTTGGG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCTTTGTGGACT20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGCTGGGGA20.1707941929974381No Hit
TACCCTTACGATGTTCCTGATTACGGTCCTCGAAGATTTCGTTGGGGACT20.1707941929974381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)