FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA22919.276094276094277No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA645.387205387205387No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT494.124579124579125No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG423.535353535353535No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA282.356902356902357No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT252.1043771043771047No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG191.5993265993265993No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG191.5993265993265993No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG181.5151515151515151No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG141.1784511784511784No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA131.0942760942760943No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA90.7575757575757576No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG80.6734006734006733No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT80.6734006734006733No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT80.6734006734006733No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG80.6734006734006733No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC70.5892255892255892No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGGGCTCGAAGATTTCGTTGG70.5892255892255892No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG70.5892255892255892No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG70.5892255892255892No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG60.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA60.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGCTCGAAGATTTCGTTG50.42087542087542085No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA50.42087542087542085No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG50.42087542087542085No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG50.42087542087542085No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA50.42087542087542085No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA40.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG40.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA40.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG40.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG40.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA40.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACTCATAATGTGCAAACAGCCCGCCTAA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT30.25252525252525254No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGTCTCTTATACACA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGACCGCCTAA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGG30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA30.25252525252525254No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.16835016835016833No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTTCAAACATCCCTCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTTGGGACTCTG20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCATAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGT20.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGTCTCGAATCTTTCGTTG20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGAGCAAACAGCCCGCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGTAGATTTCGTTGGGGA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.16835016835016833No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTTCAAACAGCCCGCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATAACGTCTAGCCATCCGATTCGTGTTAAAT20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA20.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG20.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.16835016835016833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)