FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4F06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4F06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA57134.96631965707287No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA764.654011022657685No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA694.225352112676056No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG593.6129822412737296No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT342.0820575627679117No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG271.653398652786283No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA271.653398652786283No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA231.4084507042253522No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC211.2859767299448868No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA201.224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT171.0410287813839558No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG160.9797917942437232No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA150.9185548071034906No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG140.8573178199632578No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG120.7348438456827924No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG120.7348438456827924No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG100.612369871402327No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA90.5511328842620943No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA90.5511328842620943No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG90.5511328842620943No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG80.4898958971218616No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT70.4286589099816289No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA70.4286589099816289No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT70.4286589099816289No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA70.4286589099816289No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA60.3674219228413962No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC60.3674219228413962No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG60.3674219228413962No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG50.3061849357011635No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG50.3061849357011635No Hit
TACCCTTACGATGTTCCTGATTACGCCCGGGCTCGAAGATTTCGTTGGGG40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGGCTCGAAGATTTCGTTGGG40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA40.2449479485609308No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA30.1837109614206981No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.1837109614206981No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT30.1837109614206981No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA30.1837109614206981No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA30.1837109614206981No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCCAATGCAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTAGAAGATTTCGTTGGGGACTCTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACTCATAATGTGCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGCCTCT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTGCTCGAAGATTTCGTTGGGGACTCTGTC20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGGGCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAATCAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.1224739742804654No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAACCAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTGCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA20.1224739742804654No Hit
TACCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGAGCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.1224739742804654No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAGGATTTCGTTGGGGACTCTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTCCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTTCAAACAGCCCGCCTAA20.1224739742804654No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.1224739742804654No Hit
GATGAGTCAGCGTCGAACATGCGACATTTATTGCATGATACGTGGCATTT20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTTGCAAACAGCCCGCCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTATGCATAGTGCAAACAGCCCGCCTAATG20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGTCTAA20.1224739742804654No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA20.1224739742804654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)