FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA58933.05274971941638No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA854.769921436588103No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA824.601571268237935No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA713.984287317620651No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA703.928170594837262No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA543.0303030303030303No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA492.749719416386083No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT201.122334455667789No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG170.9539842873176206No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG140.7856341189674524No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA140.7856341189674524No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA130.7295173961840629No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG120.6734006734006733No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA120.6734006734006733No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA110.6172839506172839No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA100.5611672278338945No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG90.5050505050505051No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG90.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG90.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA80.44893378226711567No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA80.44893378226711567No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA70.3928170594837262No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG70.3928170594837262No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA60.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG60.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG60.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGGGCAAACAGCCCGCCTAA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG40.22446689113355783No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.16835016835016833No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA30.16835016835016833No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGGTCCTCGAAGATTTCGTTGGGGACT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATGATGTGCAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTTCTCGAATCTTTCGTTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGAGTTTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTAATGTGCAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAATGAGC20.11223344556677892No Hit
TACCCTTACGTTGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCTCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTCA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCCTAATGTGCAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGGTTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGCTGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAACCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA20.11223344556677892No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATGAATTGCTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGACGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGAAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.11223344556677892No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTTCTGCCATCCCTCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTTCAAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACGAGCCTGGAACAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA20.11223344556677892No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.11223344556677892No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGTCTCGGCTCGAAGATTTCGTTGGGT20.11223344556677892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)