FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA37228.246013667425967No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA745.618830675778284No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG413.1131359149582387No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA372.809415337889142No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT322.4297646165527715No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA272.050113895216401No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA221.6704631738800304No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG211.5945330296127564No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG171.29081245254366No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA171.29081245254366No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG161.2148823082763858No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG151.1389521640091116No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG120.9111617312072893No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG120.9111617312072893No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC100.759301442672741No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT100.759301442672741No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA100.759301442672741No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG90.683371298405467No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA80.6074411541381929No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT80.6074411541381929No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA80.6074411541381929No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG80.6074411541381929No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA70.5315110098709187No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA60.45558086560364464No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGTCGAAGATTTCGTTG60.45558086560364464No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG50.3796507213363705No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG50.3796507213363705No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA50.3796507213363705No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTGCAAACAGCCCGCCTAA50.3796507213363705No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA50.3796507213363705No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG50.3796507213363705No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG50.3796507213363705No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA40.30372057706909644No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT40.30372057706909644No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTGCTCGAAGATTTCGTTGGGGACTCTGTC30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGCTCGAAGATTTCGTTG30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGG30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTAGTGGGCTCGAAGATTTCGTTGGGGACT30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAACCCGCCTAA30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAG30.22779043280182232No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA30.22779043280182232No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATTCAGTGCCAGCCCGCCTAA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.15186028853454822No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.15186028853454822No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTGCTGCCAGCCCGCCTAA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.15186028853454822No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTCA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTTCAAACATCCCGCCTAA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTTCAAACATCCCTCCTAA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA20.15186028853454822No Hit
TACCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACACTGGGCTCGAAGATTTCGTTGGGGA20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACATCCCGCCTAA20.15186028853454822No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA20.15186028853454822No Hit
TACCCTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAAT20.15186028853454822No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGAATTCGTTG20.15186028853454822No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.15186028853454822No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT20.15186028853454822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)