FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA987.280832095096583No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA715.274888558692422No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT564.160475482912332No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG533.937592867756315No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG402.9717682020802374No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA352.600297176820208No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG241.7830609212481425No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA171.263001485884101No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG141.040118870728083No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG141.040118870728083No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC130.9658246656760773No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG120.8915304606240713No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA100.7429420505200593No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA100.7429420505200593No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA100.7429420505200593No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT80.5943536404160475No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG80.5943536404160475No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT70.5200594353640415No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.5200594353640415No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG60.44576523031203563No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG60.44576523031203563No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA50.3714710252600297No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT50.3714710252600297No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT50.3714710252600297No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.3714710252600297No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.3714710252600297No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG50.3714710252600297No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT40.2971768202080238No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGCTTTCGTTG40.2971768202080238No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.2971768202080238No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA40.2971768202080238No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGCTCGAAGATTTCGTTGGGG40.2971768202080238No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG40.2971768202080238No Hit
GAGGAAGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.22288261515601782No Hit
GAAGAGGGCATCGTTCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCCTCGAAGATTTCGTTGGGG30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA30.22288261515601782No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA30.22288261515601782No Hit
GAGGAGGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGGCTCGAAGATTTCGTTG30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGGTGGGGA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.22288261515601782No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA30.22288261515601782No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.1485884101040119No Hit
GAGGAGGGCATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTTGGGACTCTG20.1485884101040119No Hit
GAGGAAGGCATCGTCCCCGGCGGCGGTACGGCGCTGCTGTACGCGACCAA20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA20.1485884101040119No Hit
TACCCTTACGCTGTTCCTGATTACGTTCTGGCTCGAAGATTTCGTTGGGG20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGGTGCTCGAAGATTTCTTTGTGGACT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.1485884101040119No Hit
TACCCGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGGGACT20.1485884101040119No Hit
GATGTGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTTGTTCTCGAATCTTTCGTTT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAATATTTCGTTT20.1485884101040119No Hit
GAGGAGGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.1485884101040119No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAACCCGCCTAA20.1485884101040119No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.1485884101040119No Hit
GAGGAGGGCATCGTTCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT20.1485884101040119No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG20.1485884101040119No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTT20.1485884101040119No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1485884101040119No Hit
ACGCCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT20.1485884101040119No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG20.1485884101040119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)