FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT19212.885906040268457No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA15110.134228187919463No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG1439.59731543624161No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG674.496644295302014No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT553.691275167785235No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC412.7516778523489935No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG342.2818791946308723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG291.9463087248322148No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG191.2751677852348993No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG191.2751677852348993No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG181.2080536912751678No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG151.006711409395973No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG140.9395973154362416No Hit
TACCCTTACGATGTTCCTGATCTTGGGCTCGAAGATTTCGTTGGGGACTC130.87248322147651No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA120.8053691275167786No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG120.8053691275167786No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT110.738255033557047No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG100.6711409395973155No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG80.5369127516778524No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGCTCGAAGATTTCGTTGGGG80.5369127516778524No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA80.5369127516778524No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT70.4697986577181208No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.4697986577181208No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG70.4697986577181208No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA70.4697986577181208No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG60.4026845637583893No Hit
TACCCTTACGATGTTCCTGATTACGAAGATTTCGTTGGGGACTCTGTCTC60.4026845637583893No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG60.4026845637583893No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGGGGCTCGAAGATTTCGTTG60.4026845637583893No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC60.4026845637583893No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA60.4026845637583893No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG50.33557046979865773No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA50.33557046979865773No Hit
TACCCTTACGATGTTCCTGATTACGTTGTGGGCTCGAAGATTTCGTTGGG50.33557046979865773No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG50.33557046979865773No Hit
TACCCTTACGATGTTCCTGATTACTCGAAGATTTCGTTGGGGACTCTGTC50.33557046979865773No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.2684563758389262No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2684563758389262No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2684563758389262No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG40.2684563758389262No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG40.2684563758389262No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT40.2684563758389262No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.20134228187919465No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.20134228187919465No Hit
GATGAGTCAGCGTCGCGGCGTACTTTATTTACGGCCAGAAACTCGACCTG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTTTGCTCTAAGATTTCGTT30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTGCTCGAAGATTTCGTT30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.20134228187919465No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.20134228187919465No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCTAAGATTTCGTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGGTCTCTACTATTTCGTTTGGGAC20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGTCTCGAAGATTTCGTTGGGG20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGGCTCGAAGATTTCGTTGGG20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGCTTTCGTTG20.1342281879194631No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.1342281879194631No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.1342281879194631No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT20.1342281879194631No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1342281879194631No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCCTGATACGTGTCATTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.1342281879194631No Hit
GATGAGTCAGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCTTTGGGG20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTCGATGATTTCGTTGGGGACTCTG20.1342281879194631No Hit
GATGAGTCAGCGTCGAACATACAACATTTATTACATGATACGTGGCATTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.1342281879194631No Hit
AATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGTCTCGAAGATTTCGTTGG20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTGGCTCGAAACTTTCGGTT20.1342281879194631No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGC20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGGCTCGAAGATTTCGTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTGTGCTCTAAGATTTCGTT20.1342281879194631No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTGGGGACTCTG20.1342281879194631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)