FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA89341.209044762344256No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1175.399169358560221No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1044.799261652053531No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG562.5842178126442086No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG231.0613751730503No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA221.015228426395939No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG221.015228426395939No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA200.9229349330872173No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA190.8767881864328565No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG190.8767881864328565No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT180.8306414397784956No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG170.7844946931241348No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA170.7844946931241348No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA160.7383479464697739No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA160.7383479464697739No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA150.6922011998154131No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG150.6922011998154131No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA130.5999077065066913No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG130.5999077065066913No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA120.5537609598523304No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG120.5537609598523304No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG120.5537609598523304No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT110.5076142131979695No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT110.5076142131979695No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC100.46146746654360865No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA100.46146746654360865No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA100.46146746654360865No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG100.46146746654360865No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA80.36917397323488693No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA80.36917397323488693No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGGGGCTCGAAGATTTCGTTG80.36917397323488693No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA80.36917397323488693No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG70.3230272265805261No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA70.3230272265805261No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.3230272265805261No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT70.3230272265805261No Hit
TACCCTTACGATGTTCCTGATACGCGTCTGGGGCTCGAAGATTTCGTTGG60.2768804799261652No Hit
TACCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT60.2768804799261652No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA60.2768804799261652No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT60.2768804799261652No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT50.23073373327180433No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG50.23073373327180433No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC50.23073373327180433No Hit
TACCCTTACGATGTTCCTGCTTACGCATAGTGCAAATGCAGCCCGCCTAA40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCAGCCCGCCTAA40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGCATATTGCAAATGCAGCCCGCCTAA40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG40.18458698661744347No Hit
TACCCTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT40.18458698661744347No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA40.18458698661744347No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAGTGCAGCCCGCCTAA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCTCCTAA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGT30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCATCGTGCAAATGCAGCCCGCCTAA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCATAGCGCAAATGCAGCCCGCCTAA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAACTGCAGCCCGCCTAA30.1384402399630826No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGCTTACGCATAGTGCAAATGCATCCCGCCTAA30.1384402399630826No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA30.1384402399630826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)