FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA39525.867714472822527No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1258.185985592665357No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG976.352324819908317No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT583.798297314996726No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT503.274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG352.2920759659463No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG301.9646365422396856No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA191.2442698100851344No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG171.1132940406024885No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG161.0478061558611658No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG161.0478061558611658No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT150.9823182711198428No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG150.9823182711198428No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG140.9168303863785201No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT110.7203667321545514No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT110.7203667321545514No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG90.5893909626719057No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC90.5893909626719057No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA90.5893909626719057No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG90.5893909626719057No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG80.5239030779305829No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG80.5239030779305829No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA70.45841519318926005No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG70.45841519318926005No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA70.45841519318926005No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG60.3929273084479371No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG60.3929273084479371No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG60.3929273084479371No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGGGCTCGAAGATTTCGTTGGG60.3929273084479371No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG60.3929273084479371No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGTCTCGAAGATTTCGTT50.3274394237066143No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG40.26195153896529144No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGCTTTCGTTG40.26195153896529144No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA40.26195153896529144No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGAAGATTTCGTTGGGGACTCT30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCTCTTGGGCTCGAAGATTTCGTTGG30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.19646365422396855No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA30.19646365422396855No Hit
TACCCTTACGATGTTCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGTCTCGAATATTTCGTTGT30.19646365422396855No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGCTCGAAGATTTCGTTGGG30.19646365422396855No Hit
TACCCTTACGATGTTCCTGATTACGGCTCGAAGATTTCGTTGGGGACTCT20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAATGTTTCGTTT20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGACCGCCTAA20.13097576948264572No Hit
TACCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGTCTCGAAGCTTTCGTTG20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCATCCCGCCTAA20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTTG20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGAGGCTCGAAGATTTCGTTGG20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGT20.13097576948264572No Hit
TACCCTTACGCTGTTCCTGCTTACGCATAGTGCACATGCAGCCCTCCTAA20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCAGCCCGCCTAA20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCTGTCTCTACTATTTCGTTTGGGAC20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTTCAAATGCAGCCCGCCTAA20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGGTGGGGA20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG20.13097576948264572No Hit
TACCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGAC20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGTGCTCGAATCTTTCGTTT20.13097576948264572No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.13097576948264572No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.13097576948264572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)