FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1049.683426443202979No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG1009.31098696461825No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA837.728119180633147No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG373.445065176908752No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG272.5139664804469275No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT211.9553072625698324No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT211.9553072625698324No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC151.3966480446927374No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG151.3966480446927374No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG151.3966480446927374No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT131.2104283054003724No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT90.8379888268156425No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT80.74487895716946No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG70.6517690875232774No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.6517690875232774No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG60.5586592178770949No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT50.4655493482309125No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG50.4655493482309125No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTTGGCTCGAAGATTTCGT40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTGCTCGAAGATTTCGTT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCCCGAAGATTTCGTTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGATGATTTCGTTGGG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTTTGCTCTAAGATTTCGTT20.186219739292365No Hit
TACCCTTACGCTGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.186219739292365No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA20.186219739292365No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.186219739292365No Hit
TACCCTTACGATGTTCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGAC20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTCTCGAATCTTTCGTTT20.186219739292365No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGGGCTCGAAGATTTCGTTGGG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTTTAAGATTTCGTTTGGGACTCTG20.186219739292365No Hit
TACCCTTACGCTGTTCCTGAGTACGCTCTACGATTTAGTTTGGGACTCTT20.186219739292365No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.186219739292365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACATGGCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTGGGGACTCTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGGTT20.186219739292365No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGTTCTCGAATCTTTCGTTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCCACGATTTCGTTGGGGACTCTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTTGCTCTAAGATTTCGTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTTGCTCGAAAGTTTCGGTT20.186219739292365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)