FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1277.12682379349046No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1206.7340067340067336No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG985.499438832772166No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA734.09652076318743No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA382.132435465768799No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT341.9079685746352413No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG331.8518518518518516No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA301.6835016835016834No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG231.2906846240179575No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA221.2345679012345678No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT211.1784511784511784No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG191.0662177328843996No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA191.0662177328843996No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG181.0101010101010102No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA181.0101010101010102No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG160.8978675645342313No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG100.5611672278338945No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT100.5611672278338945No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT90.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG90.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG90.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT90.5050505050505051No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA80.44893378226711567No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG80.44893378226711567No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT70.3928170594837262No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG70.3928170594837262No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.3928170594837262No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG60.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT60.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT60.33670033670033667No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT60.33670033670033667No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG50.28058361391694725No Hit
TACCCTTACGATGTTCCTGGTCTGGGCTCGAAGATTTCGTTGGGGACTCT50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA50.28058361391694725No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT40.22446689113355783No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT40.22446689113355783No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGCTCGAAGATTTCGTTGGGG40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG40.22446689113355783No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT40.22446689113355783No Hit
ACACCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG40.22446689113355783No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG40.22446689113355783No Hit
ACGCCCAAGCAGATCTACAACGATCCCGCCAACCTGTTCGTCGCCAGCTT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGTCTCGAAGATTTCGTTGT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTT30.16835016835016833No Hit
ACGCCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTTCTCGAATCTTTCGTTT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.16835016835016833No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.16835016835016833No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.16835016835016833No Hit
GAGGAAGGCATCGTTCCAGGCGGCGGTACTGCTCTTCTGCGCTCGTCCAC30.16835016835016833No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTTCGCTGT20.11223344556677892No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT20.11223344556677892No Hit
ACACCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT20.11223344556677892No Hit
ACACCCAAGCAGATCTACAACGATCCCGCCAACCTGTTCGTCGCCAGCTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGTTGTGGACTCTGCTCTTATTCGAAG20.11223344556677892No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA20.11223344556677892No Hit
GAAGAGGGAATCGTCCCCGGTGGCGGTACGGCTTTGCTGTACGCGACCAA20.11223344556677892No Hit
GAGGAGGGAATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCCCTTAGATTTCGCTGGGGA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.11223344556677892No Hit
GAGGAAGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11223344556677892No Hit
GAGGAAGGAATCGTTCCTGGCGGCGGCGTAGCCCTGCTGCGCGCTCGTGC20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGGTGGGGA20.11223344556677892No Hit
GAGGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTTGGGA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTGGGCTCGAAGATTTCGTT20.11223344556677892No Hit
GAGGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG20.11223344556677892No Hit
GAGGAGGGAATCGTCCCCGGTGGTGGTACGGCTTTGCTGTACGCGACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG20.11223344556677892No Hit
GAGGAAGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGCTTACGCTCGAAGATTTCGTTTGGGACTCTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTGGGGACTCTGT20.11223344556677892No Hit
GAAGAGGGAATCGTCCCCGGTGGCGGTACGGCGCTGCTGTACGCGACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA20.11223344556677892No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTTAACTTCCTAGTCTCAGATTTCT20.11223344556677892No Hit
GAGGAGGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCACAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT20.11223344556677892No Hit
TACCCTTAGGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAATATTTCGTTGT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTGCTCGAAGATTTCGTT20.11223344556677892No Hit
TACCCTTACGCTGTTCCTGCTTACGCTCTACGATTTCGTTTGGGACTCTT20.11223344556677892No Hit
GAAGAGGGAATCGTTCCCGGTGGCGGCACCGCGCTGCTGTACGCGACCAA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG20.11223344556677892No Hit
GAGGAGGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.11223344556677892No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG20.11223344556677892No Hit
GAGGAAGGCATCGTCCCGGGCGGCGGCACTGCGCTGCTGCGCGCCAAGAA20.11223344556677892No Hit
GAAGAAGGCATCGTTCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT20.11223344556677892No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.11223344556677892No Hit
TACCCTTACGATGTTCCTGCTTACGCTCTACGATTTCGTTTGGGACTCTT20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA20.11223344556677892No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG20.11223344556677892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)