FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG13412.641509433962264No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA13412.641509433962264No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT999.339622641509433No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT383.5849056603773586No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA333.1132075471698113No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG302.8301886792452833No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG252.358490566037736No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA171.6037735849056605No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG171.6037735849056605No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGGGCTCGAAGATTTCGTTGGG161.509433962264151No Hit
TACCCTTACGATGTTCCTGATTACGTCTCGTCTCGAAGATTTCGTTGGGG161.509433962264151No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT131.2264150943396228No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA121.1320754716981132No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG100.9433962264150944No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC100.9433962264150944No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG100.9433962264150944No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG90.8490566037735849No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT80.7547169811320755No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC70.6603773584905661No Hit
TACCCTTACGATGTTCCTGATTACGCCCGTGGGCTCGAAGATTTCGTTGG70.6603773584905661No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA60.5660377358490566No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG60.5660377358490566No Hit
TACCCTTACGATGTTCCTGATTACGCGTAGTGGCTCGAAGATTTCGTTGG50.4716981132075472No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG50.4716981132075472No Hit
TACCCTTACGATGTTCCTGATTACGTTCGTGGCTCGAAGATTTCGTTGGG50.4716981132075472No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.4716981132075472No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT40.37735849056603776No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.37735849056603776No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA40.37735849056603776No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG40.37735849056603776No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGTCTCGTCTCGAAGATTTCGTTGGGT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGGTGTGCTCGAAGATTTCGTTGGGGA30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTG30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.2830188679245283No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGATCGAAGATTTCGTTGGGGA20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTCGATGATTTCGTTGGGGACTCTG20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCGCCTGTGGGCTCGAAGATTTCGTT20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGATGGGGA20.18867924528301888No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGTTCGTGGCTCGAAGATTTTGTTGGG20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.18867924528301888No Hit
GATGAGTCAGCGTCGAACATGCTACATTTATTGCATGATACGTGGCATTT20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGATGGG20.18867924528301888No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.18867924528301888No Hit
TACCCTTACGATGCTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCGCCTCTGGGCTCGAAGATTTCGTT20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGAATCTG20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG20.18867924528301888No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCTTTGCTCTAAGATTTCGTT20.18867924528301888No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)