FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4B12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4B12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT8046.783625730994146No Hit
GATGAGTCAGCGTCGAATAGCCGTTCACTATGTTGCGGTTACTCACTGTC127.017543859649122No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG42.3391812865497075No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG42.3391812865497075No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT31.7543859649122806No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG31.7543859649122806No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT31.7543859649122806No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT31.7543859649122806No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG21.1695906432748537No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT21.1695906432748537No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATCGCATGATACGTGGCATTT21.1695906432748537No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA21.1695906432748537No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG10.5847953216374269No Hit
GATGAGTCAGCGTCGAACTGGAGAGCATCATCTGTACGTCCCGCAGCGCA10.5847953216374269No Hit
GATGAGTCAGCGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGTGGTTTCGAAGATTCCGTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGACTTTGT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCTTGGGG10.5847953216374269No Hit
GATGCGTCCGCGTCTAATAGCAGTTCACTATGTTGCGGTTACTCACTGTC10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTTCATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGAGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT10.5847953216374269No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGGATGATACGTTGCATTT10.5847953216374269No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.5847953216374269No Hit
TACCCTTACGATGTTCTGATTACGCGGGCTTGAAGCTTTCGTTGGGGACT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAAGATTTCGTTGGG10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGTCTCGAAGATTTCGTTGT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG10.5847953216374269No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGGCGAATAGCCGTTCACTATGTTGCGTTTACTCACTGTC10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTACATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGTCGAACAAGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGTCTCGAAGATTTCGTTG10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGTCGAACAGGCTGCACCGAACGACTTCTTCGACAACCCG10.5847953216374269No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATTTCATGATACGTGTCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGTG10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGCTGGG10.5847953216374269No Hit
GATGCGTCCGCGTCTAACATGAACCTGTATTGCATTATACTTGGCCTTTC10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT10.5847953216374269No Hit
GATGAGTCAGCGTCGAATAGCCGTTCACTATGTTGCGGTTACTCACTTTC10.5847953216374269No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCCTTT10.5847953216374269No Hit
TACCCTTATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTGTCT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGCTCGAGGATTTGGTTGT10.5847953216374269No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGAGGCTCGAAGATTTCGTTGG10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTAGGGA10.5847953216374269No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTTCATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGTCGACCATGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
GATGCGTCAGCGACGAACATGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCACGAAGATTTCGTTGGGGAC10.5847953216374269No Hit
GATGCGTCCGCGTCAAACATGCACCATTTATTGCATGATACCTGGCATTT10.5847953216374269No Hit
GATGCGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
GATGCGTCCGCGTCTAATAGCCGTTCACTATGTTGCGGTTACTCACTGTC10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT10.5847953216374269No Hit
GATGAGTCAGCGTCGGACATGCAACATTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT10.5847953216374269No Hit
GATGCGTCCGCGTCTAACATGCACCCTCTATTGCATGATACGTGGCATTT10.5847953216374269No Hit
GATGAGTCAGCGTCGAATAGCCGTTCACTATGTTGCGGTTACTCACATCT10.5847953216374269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)